Repository of Research and Investigative Information

Repository of Research and Investigative Information

دانشگاه علوم پزشکی و خدمات بهداشتی درمانی زنجان

shRNA-mediated downregulation of alpha-N-Acetylgalactosaminidase inhibits migration and invasion of cancer cell lines

(2017) shRNA-mediated downregulation of alpha-N-Acetylgalactosaminidase inhibits migration and invasion of cancer cell lines. Iranian Journal of Basic Medical Sciences. pp. 1029-1036. ISSN 2008-3866

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Abstract

Objective(s): Extracellular matrix (ECM) is composed of many kinds of glycoproteins containing glycosaminoglycans (GAGs) moiety. The research was conducted based on the N-Acetylgalactosamine (GalNAc) degradation of ECM components by alpha-N-acetylgalactosaminidase (Nagalase) which facilitates migration and invasion of cancer cells. This study aims to investigate the effects of Naga-shRNA downregulation on migration and invasion of cancer cell lines. Materials and Methods: In this study, MCF-7 cell line (human mammary carcinoma cell line) and A2780 (human ovarian carcinoma cell line) were used. The level of normalized Naga expression and Nagalase protein were evaluated by quantitative polymerase chain reaction and enzyme-linked immunosorbent assay/western blotting, respectively. Migration and invasion were determined using transwell assays, and statistical analysis was carried out by ANOVA test. Results: Response to transduction by shRNA compared to the control group, migrative and invasive properties of the transfected cells were significantly inhibited. Conclusion: These results indicate that Nagalase may have an important role in migration and invasion of cancer cells and can be considered as a candidate for further studies.

Item Type: Article
Keywords: Alpha-N-Acetylgalactosaminidase Cancer Extracellular matrix Invasion Migration shRNA MACROPHAGE-ACTIVATING FACTOR BREAST-CANCER EXTRACELLULAR-MATRIX PANCREATIC-CANCER ASSAY SYSTEM GLYCOSYLATION EXPRESSION BINDING DEFICIENCY CARCINOMA Medicine, Research & Experimental Pharmacology & Pharmacy
Page Range: pp. 1029-1036
Journal or Publication Title: Iranian Journal of Basic Medical Sciences
Abstract and Indexing: ISI, Pubmed, Scopus
Quartile : Q4
Volume: 20
Number: 9
Identification Number: <Go to ISI>://WOS:000409212700009
ISSN: 2008-3866
Depositing User: خانم مریم زرقانی
URI: http://repository.zums.ac.ir/id/eprint/98

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