Repository of Research and Investigative Information

Repository of Research and Investigative Information

دانشگاه علوم پزشکی و خدمات بهداشتی درمانی زنجان

Generation of Insulin-Producing Cells From Human-Induced Pluripotent Stem Cells Using a Stepwise Differentiation Protocol Optimized With Platelet-Rich Plasma

(2017) Generation of Insulin-Producing Cells From Human-Induced Pluripotent Stem Cells Using a Stepwise Differentiation Protocol Optimized With Platelet-Rich Plasma. Journal of Cellular Physiology. pp. 2878-2886. ISSN 0021-9541

Full text not available from this repository. (Request a copy)

Official URL: http://apps.webofknowledge.com/full_record.do?prod...

Abstract

Studies on patient-specific human-induced pluripotent stem cells (hiPSCs) as well as a series of autologous growth factors presumably will reveal their many benefits for cell base replacement therapy in type 1 diabetes mellitus (TIDM) patients in the future. For this purpose, we established a multistep protocol by adding platelet-rich plasma (PRP) that induce the hiPSCs into insulin-producing cells (IPCs). Wepresent here a differentiation protocol consisting of five stages in two groups including protocol with PRP and without PRP. Characteristics of derived IPCs in both groups were evaluated at the mRNA and protein levels, cell cycle and viability in the end stage of cell differentiation. The in vitro studies indicated the treatment of hiPSCs in the protocol with PRP resulting in differentiated cells with strong characteristics of IPCs including islet-like cells, the expression of mature and functional pancreatic beta cell specific marker genes. In addition to these pancreatic specific markers were detected by immunochemistry and Western blot. Our differentiated cells in two groups secreted insulin and C-peptide in a glucose stimulation test by ELISA showing in vitro functional. The results of the cell cycle assay confirmed that differentiation has been done. We reported for the first time that PRP might be ideal additive in the culture medium to induce pancreatic differentiation in the hiPSCs. This study provides a new approach to investigate the role of PRP in pancreatic differentiation protocols and enhance the feasibility of using patient-specific iPSCs and autologous PRP for future beta cells replacement therapies for T1DM.

Item Type: Article
Keywords: PANCREATIC BETA-CELLS IN-VITRO DIFFERENTIATION HEPATOCYTE GROWTH-FACTOR PROLIFERATION PROMOTES OVEREXPRESSION INDUCTION ISLETS MICE Cell Biology Physiology
Page Range: pp. 2878-2886
Journal or Publication Title: Journal of Cellular Physiology
Abstract and Indexing: ISI, Pubmed, Scopus
Quartile : Q2
Volume: 232
Number: 10
Identification Number: https://doi.org/10.1002/jcp.25721 <Go to ISI>://WOS:000407019900029
ISSN: 0021-9541
Depositing User: خانم مریم زرقانی
URI: http://repository.zums.ac.ir/id/eprint/89

Actions (login required)

View Item View Item