Repository of Research and Investigative Information

Repository of Research and Investigative Information

دانشگاه علوم پزشکی و خدمات بهداشتی درمانی زنجان

Determination of carcinoembryonic antigen as a tumor marker using a novel graphene-based label-free electrochemical immunosensor

(2021) Determination of carcinoembryonic antigen as a tumor marker using a novel graphene-based label-free electrochemical immunosensor. Analytical Biochemistry. ISSN 00032697

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Official URL: https://www.scopus.com/inward/record.uri?eid=2-s2....

Abstract

In this work, a simple label-free electrochemical immunosensor for the detection of carcinoembryonic antigen (CEA) was developed. At first, the GC electrode was coated with partially reduced graphene oxide (rGO) to form a platform to bind the antibody. After activating the carboxyl groups of rGO through the EDC/NHS linker, the electrode surface was covered with the antibody. Then, the electrochemical behavior of the antibody-modified electrode and the parameters of the interactions of antibody-antigen immune complexes were investigated using cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). This immune-complex layer was found to attenuate the electrochemical current which can be used as a good signal to determine the antigen concentration. The proposed immunosensor exhibited a good amperometric response to CEA within a concentration range of 0.1�5 ng mL�1 with a detection limit of 0.05 ng ml�1. Furthermore, the developed method was evaluated for the detection of CEA in the real sample (human blood serum), and the results were comparable with the reference values obtained by the standard enzyme-linked immunosorbent assay (ELISA). Our findings suggest the present immunosensor as a good candidate for application in clinical screening. © 2020 Elsevier Inc.

Item Type: Article
Keywords: Antibodies; Antigens; Cyclic voltammetry; Diagnosis; Electrochemical impedance spectroscopy; Graphene; Immunosensors; Reduced Graphene Oxide; Tumors, Amperometric response; Carcinoembryonic antigen; Concentration ranges; Electrochemical behaviors; Electrochemical currents; Electrochemical immunosensors; Enzyme linked immunosorbent assay; Reduced graphene oxides (RGO), Electrochemical electrodes
Subjects: QU Biochemistry. Cell Biology and Genetics > QU 100-133 Biochemistry of the Human Body
Divisions: Education Vice-Chancellor Department > Faculty of Medicine > Department of Basic Science > Department of Clinical Biochemistry
Journal or Publication Title: Analytical Biochemistry
Abstract and Indexing: ISI, Pubmed, Scopus
Quartile : Q2
Volume: 613
Publisher: Academic Press Inc.
Identification Number: https://doi.org/10.1016/j.ab.2020.114017
ISSN: 00032697
Depositing User: خانم فائزه مظفری
URI: http://repository.zums.ac.ir/id/eprint/7967

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