Repository of Research and Investigative Information

Repository of Research and Investigative Information

دانشگاه علوم پزشکی و خدمات بهداشتی درمانی زنجان

Anti-proliferative properties, biocompatibility, and chemical composition of different extracts of plantago major medicinal plant

(2021) Anti-proliferative properties, biocompatibility, and chemical composition of different extracts of plantago major medicinal plant. Iranian Biomedical Journal. pp. 106-116. ISSN 1028852X

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Abstract

Background: To study the anticancer activity of Plantago major, we assessed the effect of ethanolic, methanolic and acetonic extracts of this plant on HCT-116, SW-480, and HEK-293 cell lines as control. Methods: The cytotoxic activity, biocompatibility, and toxicity were evaluated by MTT assay, hemolysis, and Artemia salina-LD50 (on mice) tests, respectively. The analysis of extracts compounds was performed by GC-MS analysis. Results: The results showed that all the extracts had the most antiproliferative properties on the HCT-116 cell line. The root extract of P. major was more effective than the aerial parts, and IC50 value of ethanolic, methanolic and acetonic root extracts was 405.59 µg/mL, 470.16 µg/mL, and 82.26 µg/mL, respectively on HCT-116 cell line at 72 h. Hemolysis degree of the ethanolic extract of P. major aerial and root parts were approximately 1 at 400 μg/mL. No significant interference of the RBC was observed. The ethanolic extracts, the Artemia survived every concentration used, and no toxicity was observed. One week after oral administration of different parts of P. major extracts, none of the mice died, even the mice preserved with 2000 mg/kg. These results of GC/MS analysis showed that P. major extracts contain potential anticancer compounds, such as stearic acid (8.61) in aerial parts of methanolic extract and 1,2-Benzenedicarboxylic acid, mono(2-ethylhexyl)ester (88.07 and 40.63) in aerial and root parts of acetonic extract of P. major. Conclusions: Our findings suggest that the P. major is a source of important pharmaceutical compounds with antiproliferative properties. © 2021, Pasteur Institute of Iran. All rights reserved.

Item Type: Article
Keywords: Gas chromatography-mass spectrometry; HCT-116 cells; Hemolysis; Lethal dose 50
Divisions: Education Vice-Chancellor Department > Faculty of Pharmacy > Department of Pharmacognosy and Pharmaceutical Biotechnology
Page Range: pp. 106-116
Journal or Publication Title: Iranian Biomedical Journal
Abstract and Indexing: Pubmed, Scopus
Quartile : Q2
Volume: 25
Number: 2
Publisher: Pasteur Institute of Iran
Identification Number: https://doi.org/10.29252/ibj.25.2.106
ISSN: 1028852X
Depositing User: خانم فائزه مظفری
URI: http://repository.zums.ac.ir/id/eprint/7949

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