Repository of Research and Investigative Information

Repository of Research and Investigative Information

دانشگاه علوم پزشکی و خدمات بهداشتی درمانی زنجان

Poly(caprolactone)-poly(ethylene glycol)-poly(caprolactone) (PCL-PEG-PCL) nanoparticles: A valuable and efficient system for in vitro and in vivo delivery of curcumin

(2016) Poly(caprolactone)-poly(ethylene glycol)-poly(caprolactone) (PCL-PEG-PCL) nanoparticles: A valuable and efficient system for in vitro and in vivo delivery of curcumin. Rsc Advances. pp. 14403-14415.

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Abstract

Among the potent anticancer agents, curcumin is known as an effective agent against many different types of cancer cells. Its clinical applications have been limited because of hydrophobicity, low gastrointestinal absorption and fast metabolism. In the present study, curcumin was encapsulated within PCL-PEG-PCL micelles through a single-step nano-precipitation method, leading to the creation of CUR/PCL-PEG-PCL micelles. The PCL-PEG-PCL copolymer was characterized in vitro using 1H NMR, FTIR, DSC and GPC techniques. PCL-PEG-PCL copolymers with curcumin were self-assembled into micelles in aqueous solution. The resulting micelles were characterized further using various techniques such as DLS and AFM. The anti-tumor properties of curcumin-loaded micelles were evaluated in cancer cell lines both in vitro and in vivo. The results showed the successful formation of spherical curcumin-loaded micelles. The encapsulation efficiency of curcumin was 83 ± 1.29. The results of AFM revealed that the micelles have spherical shapes with a size of 70.34 nm. In vitro release of curcumin from curcumin-entrapped micelles followed a remarkably sustained release profile. The curcumin-loaded micelle showed an efficient physical stability and a high loading efficiency. The results of in vivo experiments indicated that the curcumin-loaded micelles significantly reduce the tumor size, prolong the survival and increase splenocyte proliferation and IFN-γ production as well as significantly decreasing the IL-4 production. The results indicate that the successful formulation of curcumin loaded PCL-PEG-PCL micelles had a significant chemopreventive effect on cancer cell lines through cell proliferation inhibition, apoptosis induction and anti-tumor immunity stimulation. © 2016 The Royal Society of Chemistry.

Item Type: Article
Keywords: Cell culture Cell death Cell proliferation Cells Cytology Diseases Efficiency Encapsulation Micelles Polyethylene oxides Precipitation (chemical) Solutions Tumors Apoptosis induction Chemopreventive effects Clinical application Encapsulation efficiency Gastrointestinal absorptions In-vivo experiments Proliferation inhibition Splenocyte proliferation Polyethylene glycols
Page Range: pp. 14403-14415
Journal or Publication Title: Rsc Advances
Abstract and Indexing: ISI, Pubmed
Quartile : Q2
Volume: 6
Number: 17
Identification Number: https://doi.org/10.1039/c5ra24942b
Depositing User: خانم مریم زرقانی
URI: http://repository.zums.ac.ir/id/eprint/789

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