Repository of Research and Investigative Information

Repository of Research and Investigative Information

دانشگاه علوم پزشکی و خدمات بهداشتی درمانی زنجان

In vitro regeneration and transient expression of recombinant sesquiterpene cyclase (SQC) in Artemisia annua L

(2016) In vitro regeneration and transient expression of recombinant sesquiterpene cyclase (SQC) in Artemisia annua L. South African Journal of Botany. pp. 225-231.

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Abstract

In the present study, we developed an efficient and reliable protocol for in vitro plant regeneration and transient gene expression in medicinal plant Artemisia annua L. For in vitro regeneration, leaf explants were cultivated in MS medium supplemented by combination of plant growth regulators including α-naphthalene-acetic acid, 6-benzyl-aminopurine, indole-3-acetic acid and 2,4-dichlorophenoxyacetic acid. The highest frequency of shoot organogenesis occurred on MS medium supplemented with 0.5 mg/l NAA plus 2 mg/l BA (86.6) and MS medium supplemented with 1 mg/l IAA plus 2 mg/l BA (80). Root induction was obtained on MS medium supplemented with 0.1 mg/l IBA. This is a simple, reliable, rapid and high efficient regeneration system for A. annua L. in short period via adventitious shoot induction approach. Based on the sequence of a putative sesquiterpene cyclase from A. annua, 1.7 kb fragment was amplified by PCR from Iranian variety of this plant after cDNA synthesis. Sesquiterpene cyclase gene was used for sub-cloning into pBI121 between XbaI and SacI sites to allow the expression of the gene driven by CaMV35S promoter. The recombinant plasmids were transferred to competent cells of Agrobacterium tumefaciens strain GV 3850 for vacuum agro-infiltration. HPLC results showed 30.09 increase in atremisinin content (0.15) in the agroinfiltrated samples (0.67) in comparison to the control (0.51). © 2016 South African Association of Botanists.

Item Type: Article
Keywords: Artemisia annua L Recombinant sesquiterpene cyclase Regeneration Transient expression dicotyledon enzyme activity gene expression leaf medicinal plant mitochondrial DNA plasmid root shoot terpene Agrobacterium tumefaciens Artemisia annua
Page Range: pp. 225-231
Journal or Publication Title: South African Journal of Botany
Abstract and Indexing: ISI
Quartile : Q2
Volume: 104
Identification Number: https://doi.org/10.1016/j.sajb.2015.10.005
Depositing User: خانم مریم زرقانی
URI: http://repository.zums.ac.ir/id/eprint/763

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