Repository of Research and Investigative Information

Repository of Research and Investigative Information

دانشگاه علوم پزشکی و خدمات بهداشتی درمانی زنجان

Thin Cell Layer, a Suitable Explant for In vitro Regeneration of Saffron (Crocus sativus L.)

(2017) Thin Cell Layer, a Suitable Explant for In vitro Regeneration of Saffron (Crocus sativus L.). Journal of Agricultural Science and Technology. pp. 1429-1435. ISSN 1680-7073

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Abstract

Saffron (Crocus sativus L.) is a sterile species and biotechnological method is suggested to improve different characteristic in this valuable plant. In this study, an efficient protocol was provided for callus induction and regeneration of saffron using thin cell layer explants. Longitudinally and transversally, thin cell layer explants with approximately 1 mm thickness of apical buds were cultured on MS medium supplemented with different concentrations of 2,4-Dichlorophenoxyacetic acid (2,4-D), 6-BenzylAminoPurine (BAP) and 1-NaphthaleneAcetic Acid (NAA). The highest amount of callus induction (100) was obtained from transverse thin cell layer explants of apical bud in MS medium containing 2 mg L-1 BAP and 2 mg L-1 NAA during 3 months incubation under dark condition at 20 degrees C. The maximum percent of shoot regeneration (75) was observed on the MS medium containing 0.5 mg L-1 BAP. The results of this investigation revealed that the thin cell layers from buds are suitable explants for regeneration.

Item Type: Article
Keywords: Callus induction Tissue culture Growth Regulators Shoot induction ARABIDOPSIS-THALIANA TISSUE-CULTURE MICROPROPAGATION PLANT Agriculture, Multidisciplinary
Page Range: pp. 1429-1435
Journal or Publication Title: Journal of Agricultural Science and Technology
Abstract and Indexing: ISI, Scopus
Quartile : Q2
Volume: 19
Number: 6
Identification Number: <Go to ISI>://WOS:000413291400018
ISSN: 1680-7073
Depositing User: خانم مریم زرقانی
URI: http://repository.zums.ac.ir/id/eprint/75

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