Repository of Research and Investigative Information

Repository of Research and Investigative Information

دانشگاه علوم پزشکی و خدمات بهداشتی درمانی زنجان

Evaluation of hypoxia inducible factor-1 alpha gene expression in colorectal cancer stages of Iranian patients

(2016) Evaluation of hypoxia inducible factor-1 alpha gene expression in colorectal cancer stages of Iranian patients. Journal of Cancer Research and Therapeutics. pp. 1313-1317.

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Abstract

Aim of Study: Colorectal cancer (CRC) is the fourth most prevalent cancer globally. Several factors have roles in cancer establishment. One of the most important factors is hypoxia that induces hypoxia inducible factor-1 (HIF-1). The HIF-1 alpha overexpressed in hypoxia conditions and plays a pivotal role in carcinogenesis features. In this study, we aimed to examine the efficiency of HIF-1 alpha gene expression at mRNA and protein's level for CRC diagnosing and staging. Materials and Methods: In this study, the cases included into 75 cancer specimens in different stages (Group 2 = Stage 1, Group 3 = Stage 2, and Group 4 = Stage 3, 4) and ten normal specimens as control (Group 1). Real-Time reverse transcription-polymerase chain reaction and immunohistochemistry (IHC) were performed for measuring gene expression at RNA and protein's level, respectively. The raw data were analyzed in the SPSS20 software. Results: HIF-1 alpha gene expression rate (2-ΔΔCT) and ΔCT values were significantly higher increased in Group 4 in compare to control (P < 0.001). Other cancer groups (2 and 3) had greater ΔCT values than control, but it was not statistically significant. Moreover, the rate of HIF-1 alpha gene expression (2-ΔΔCT) was increased with cancer stages. According to the IHC results, there was a positive relationship between CRC stages and HIF-1 alpha protein expression (P < 0.05). Conclusions: HIF-1 alpha gene expression increased in earlier up to metastasis stages of CRC, but the assessment of HIF-1 alpha gene expression has not important role in the diagnosis of cancer in early stages and classification of carcinoma because the increasing of HIF-1 alpha gene expression is not significant in early cancer stages.

Item Type: Article
Keywords: Cancer and diagnosis colorectal hypoxia inducible factor-1 alpha gene beta actin hypoxia inducible factor 1alpha tumor marker agar gel electrophoresis Article cancer classification cancer diagnosis cancer staging colorectal cancer controlled study gene expression human human tissue immunohistochemistry Iranian (citizen) protein determination protein function reverse transcription polymerase chain reaction signal transduction colorectal tumor female genetics Iran male metabolism pathology polymerase chain reaction Biomarkers, Tumor Colorectal Neoplasms Humans Hypoxia-Inducible Factor 1, alpha Subunit Neoplasm Staging
Page Range: pp. 1313-1317
Journal or Publication Title: Journal of Cancer Research and Therapeutics
Abstract and Indexing: ISI, Pubmed, Scopus
Quartile : Q4
Volume: 12
Number: 4
Identification Number: https://doi.org/10.4103/0973-1482.199542
Depositing User: خانم مریم زرقانی
URI: http://repository.zums.ac.ir/id/eprint/740

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