Repository of Research and Investigative Information

Repository of Research and Investigative Information

دانشگاه علوم پزشکی و خدمات بهداشتی درمانی زنجان

Synthesis and Investigation of the Curcumin-Loaded Magnetic Lipid Nanoparticles and Their Cytotoxicity Assessment on Human Breast Carcinoma Cell Line

(2020) Synthesis and Investigation of the Curcumin-Loaded Magnetic Lipid Nanoparticles and Their Cytotoxicity Assessment on Human Breast Carcinoma Cell Line. Jundishapur Journal of Natural Pharmaceutical Products. ISSN 1735-7780

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Official URL: https://www.scopus.com/inward/record.uri?eid=2-s2....

Abstract

Chemotherapy fails to eradicate cancer cells, mainly due to the lack of selective drug accumulation into the tumor site, which can affect healthy cells, as well. In this research, we studied the magnetite nanostructured lipid carriers (NLCs) for targeted delivery of curcumin into breast cancer cells. Superparamagnetic iron oxide nanoparticles (SPIONs) were prepared using coprecipitation method by mixing FeCl2 and FeCl3 in a suitable ratio in alkaline media. The resultant ferrofluid was very stable and possessed high magnetic properties. To prepare SPIONs containing NLCs (NLC-SPIONs), cetyl palmitate and cod-liver oil, Tween 80 and span60 were used as solid lipid, liquid lipid, surfactant, and co-surfactant, respectively. Curcumin as an anticancer drug was loaded into NLC-SPIONs (CUR-NLC-SPIONs) and its characteristics, including particle size, zeta potential, polydispersity index (PDI), drug entrapment efficacy, drug-loading capacity, and thermal stability were evaluated. The results showed that CUR-NLC-SPIONs had a mean particle size of 166.7 ±14.20 nm, mean zeta potential of -27.6 ±3.83 mv, and PDI of 0.24 ±0.14. Encapsulation efficiency for all prepared nanoparticles (NPs) was 99.95 ± 0.015% and drug-loading capacity was 3.76 ± 0.005%. Morphological studies were carried out by transmission electron microscopy (TEM) indicating spherical morphology of the NPs. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay measurements on cell viability proved that the synthesized CUR-NLC-SPIONs possess a better cytotoxic activity against human breast cancer cells compared with free curcumin. This new drug delivery system, which benefits from superparamagnetic properties may serve as a suitable platform for developing new biocompatible drug carriers and with a potential to use in targeted cancer therapy. Copyright © 2020, Author(s). This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.

Item Type: Article
Keywords: Article,Cancer,Curcumin,IC50,MCF-7 cell line,MTT assay,Nanostructure Lipid Carrier,Superparamagnetic,Targeted Drug Delivery,breast carcinoma cell line,cell viability,controlled study,dispersity,drug cytotoxicity,drug delivery system,drug release,drug solubility,drug synthesis,human,human cell,nanoencapsulation,particle size,photon correlation spectroscopy,superparamagnetic iron oxide nanoparticle,transmission electron microscopy,zeta potential
Subjects: QU Biochemistry. Cell Biology and Genetics > QU 300-560 Cell Biology and Genetics
QZ Pathology > QZ 200-380 Neoplasms
Divisions: Education Vice-Chancellor Department > Faculty of Pharmacy > Department of Toxicology and Pharmacology
Journal or Publication Title: Jundishapur Journal of Natural Pharmaceutical Products
Abstract and Indexing: ISI, Scopus
Volume: 15
Number: 2
Publisher: Kowsar Medical Publishing Company
Identification Number: https://doi.org/10.5812/jjnpp.91886
ISSN: 1735-7780
ISBN: 17357780 (ISSN)
Depositing User: خانم فائزه مظفری
URI: http://repository.zums.ac.ir/id/eprint/7169

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