Repository of Research and Investigative Information

Repository of Research and Investigative Information

دانشگاه علوم پزشکی و خدمات بهداشتی درمانی زنجان

Effect of Biotin-Targeted Protein-Based Nanoparticles Contain of Curcumin on the Expression of Apoptotic Index Bax and Bcl2 Proteins

(2020) Effect of Biotin-Targeted Protein-Based Nanoparticles Contain of Curcumin on the Expression of Apoptotic Index Bax and Bcl2 Proteins. Journal of Polymers and the Environment. ISSN 1566-2543

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Official URL: https://link.springer.com/article/10.1007/s10924-0...

Abstract

In this study, biotin functionalized bovine serum albumin (BSA-biotin) loaded with curcumin (CUR), was synthesized and evaluated as drug carrier. The aim of this study is evaluation of the curcumin induced apoptosis related proteins, Bcl-2 and Bax expression via targeted albumin based nanoparticles. The synthesized BSA-biotin-CUR was characterized by different techniques such as: TEM, UVâ��Vis, FTIR, and DLS. The loading efficiency of CUR was 7.9%. The result indicates that the particles were measured around 185.4 nm with a zeta potential of -16.8 mV. Cytotoxicity results on mouse mammary tumor cell line (4T1) confirmed that formulated CUR, BSA-biotin-CUR, has high anticancer effect compared to free CUR. Also, real-time PCR method revealed that BSA-biotin-CUR increased Bax protein expression, and also downregulated Bcl-2 protein in 4T1 cells, which proven the ability of synthesized nanoparticles. We suggest that BSA-biotin-CUR can improve therapeutic effect, of CUR so that it can overcome the limitations of CUR with a great potential for further evaluations and applications. © 2020, Springer Science+Business Media, LLC, part of Springer Nature.

Item Type: Article
Keywords: Anticancer effects,BSA,Bax,Bcl2,Biosynthesis,Bovine serum albumins,Cell culture,Cell death,Coenzymes,Curcumin,Induced apoptosis,Loading efficiency,Mammals,Nanoparticles,Polymerase chain reaction,Protein expressions,Real-time PCR method,Target delivery,Targeted drug delivery,Targeted proteins,Therapeutic effects
Subjects: QU Biochemistry. Cell Biology and Genetics > QU 300-560 Cell Biology and Genetics
QZ Pathology > QZ 200-380 Neoplasms
Divisions: Education Vice-Chancellor Department > Faculty of Pharmacy > Department of Pharmacotherapy
Journal or Publication Title: Journal of Polymers and the Environment
Abstract and Indexing: ISI, Scopus
Quartile : Q2
Publisher: Springer
Identification Number: https://doi.org/10.1007/s10924-020-01831-7
ISSN: 1566-2543
ISBN: 15662543 (ISSN)
Depositing User: خانم فائزه مظفری
URI: http://repository.zums.ac.ir/id/eprint/7130

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