Repository of Research and Investigative Information

Repository of Research and Investigative Information

دانشگاه علوم پزشکی و خدمات بهداشتی درمانی زنجان

In-vitro study of hottentotta schach crude venom anticancer effects on mcf-7 and vero cell lines

(2018) In-vitro study of hottentotta schach crude venom anticancer effects on mcf-7 and vero cell lines. Iranian Journal of Pharmaceutical Research. pp. 192-202. ISSN 17350328

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Official URL: http://ijpr.sbmu.ac.ir/article_1100957_af29281e366...

Abstract

Scorpion venoms contain potentially useful pharmacological agents. Several studies demonstrate that the venoms of some scorpions induce apoptosis and inhibit the growth of cancer cells; therefore, they have been investigated for isolating anticancer components. In this study, antitumor effects of Hottentotta schach crude venom on MCF-7 (breast cancer cell line) as test group and Vero (African green monkey kidney normal cell line) as control group were analyzed. Cell toxicity was analyzed using MTT and neutral red (NR) uptake assays and apoptosis induction was analyzed using comet assay and caspase-3 activity. Oxidative stress following Hottentotta schach crude venom treatment was analyzed using nitrite oxide (NO) determination assay, reduced glutathione (GSH) and catalase enzyme activity assays. Results showed that crude venom (25-200 μg/mL) induced apoptosis and inhibited the growth of MCF-7 and to a lesser extent in Vero cell lines. Nitrite oxide concentration increased while glutathione concentration and catalase enzyme activity were decreased in MCF-7 cells; however, results in Vero cells were reversed completely. It can be concluded that Hottentotta schach crude venom disturbs the oxidation and reduction potential in cancer cells and ultimately induce apoptosis. So this venom can be used as a good source for isolation and designing new anticancer drugs. © 2020, Iranian Journal of Pharmaceutical Research. All rights reserved.

Item Type: Article
Keywords: Anti-cancer drug; Cancer therapy; Hottentotta schach; Scorpion crude venom
Subjects: QV Pharmacology > QV 243-269 Anti-Inflammatory Agents. Anti-Infective Agents. Antineoplastic Agents
QZ Pathology > QZ 200-380 Neoplasms
Divisions: vice research > Journal of Advances in Medical and Biomedical Research
Page Range: pp. 192-202
Journal or Publication Title: Iranian Journal of Pharmaceutical Research
Abstract and Indexing: ISI, Scopus, Other
Quartile : Q4
Volume: 19
Number: 1
Publisher: Shahid Beheshti University of Medical Sciences(Sbum)
Identification Number: https://doi.org/10.22037/ijpr.2020.1100957
ISSN: 17350328
Depositing User: خانم فائزه مظفری
URI: http://repository.zums.ac.ir/id/eprint/6988

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