Repository of Research and Investigative Information

Repository of Research and Investigative Information

دانشگاه علوم پزشکی و خدمات بهداشتی درمانی زنجان

Harnessing nanoparticles for the efficient delivery of the CRISPR/Cas9 system

(2020) Harnessing nanoparticles for the efficient delivery of the CRISPR/Cas9 system. Nano Today. ISSN 17480132 (ISSN)

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Official URL: https://www.sciencedirect.com/science/article/abs/...

Abstract

Exploiting bacterial DNA-acting enzymes and expanding their repertoire for genome engineering have been major technological and conceptual advances in molecular biology over the last decade. The CRISPR-Cas9 system offers many attractive superiorities, such as multiplexing, high precision, low cost, and simplicity compared to other strategies/systems/approaches known to date for gene editing. The efficient co-delivery of Cas9 and single guide RNA(s) into a desired cell and subsequent correct targeting of selected genomic fragment(s) are among the most critical and determining issues for CRISPR-Cas9-based genome engineering. CRISPR/Cas9 components can be transported into target cells via various delivery methods, including physical methods (such as electroporation and microinjection) as well as viral and non-viral methods. Physical and viral methods, with all their privileges, still suffer from disadvantages including induction of immune responses, cell damage, lack of high specificity, etc. We are witnessing a remarkable increase in the employment of nanomaterials as non-viral carriers for the delivery of the CRISPR/Cas9 system. Nanoparticles have so far presented numerous advantages such as ease of synthesis, high efficiency, low cost, size tunability, non-mutagenicity, non-immunogenicity, etc. with regard to the delivery of CRISPR/Cas9. Here, we will review the recent progress in the delivery of CRISPR/Cas9 system components via nanomaterials and outline future challenges. © 2020 Elsevier Ltd

Item Type: Article
Keywords: CRISPR/Cas9,Cell engineering,Costs,Delivery,Delivery methods,Future challenges,Genome editing,Genome engineering,Genomic fragments,High specificity,Molecular biology,Nanoparticles,Nanostructured materials,Non-viral carriers,Non-viral vector,Physical methods,Synthesis (chemical),System components
Subjects: QU Biochemistry. Cell Biology and Genetics > QU 300-560 Cell Biology and Genetics
Divisions: Education Vice-Chancellor Department > Faculty of Pharmacy > Department of Pharmacognosy and Pharmaceutical Biotechnology
Journal or Publication Title: Nano Today
Abstract and Indexing: ISI, Scopus, Other
Quartile : Q1
Volume: 34
Publisher: Elsevier B.V.
Identification Number: https://doi.org/10.1016/j.nantod.2020.100895
ISSN: 17480132 (ISSN)
Depositing User: خانم فائزه مظفری
URI: http://repository.zums.ac.ir/id/eprint/6951

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