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دانشگاه علوم پزشکی و خدمات بهداشتی درمانی زنجان

Genotyping of candida albicans strains obtained from oropharyngeal candidiasis patients based on abc and rps typing systems

(2020) Genotyping of candida albicans strains obtained from oropharyngeal candidiasis patients based on abc and rps typing systems. Jundishapur Journal of Microbiology. pp. 1-6. ISSN 20083645 (ISSN)

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Background: Candida albicanss has been introduced as one of the most common causes of nosocomial infections. Molecular typing methods are powerful tools in epidemiology to investigate the infection source of candidiasis, identify the transmission routes, and control the measures. Objectives: This study aimed for genotyping C. albicans species isolated from oral cavities of the non-HIV patients who suffer from oropharyngeal candidiasis via combined ABC and repeat sequences (RPS) typing systems. Methods: In this study, 31 DNA samples of clinical isolates of C. albicans were evaluated in terms of 25s ribosomal DNA region sequence or ABC typing, and ALT repeats numbers within RPS. DNA was amplified in two separate reactions, and the PCR products were electrophoresed to identify the genotypes of the isolates. Based on the band's pattern, phylogenetic analysis was conducted by UPGMA, and the discriminatory power of ABC and RPS typing was measured by Simpson's index of diversity. Results: Genotype A with (14 isolates, 45.2%) were the most frequent and followed by genotype B (10 isolates, 32.3%) and Genotype C (7 isolates, 22.6%), respectively. In addition, genotype 3 with 25 isolates (80.6%) were the most prevalent, followed by genotype 2/3 (4 isolates, 12.9%) and genotype 3/4 (2 isolates, 6.5%) respectively. No significant relationship was found between the obtained genotypes and drug-resistant isolates (P \textless 0.05). Conclusions: This study showed that 25s rDNA and RPS typing is a quick, simple, and cost-effective method with average discriminatory power and good reproducibility for C. albicans genotyping. It can be used for the epidemiology of C. albicans infections. © 2020, Author(s).

Item Type: Article
Keywords: Article,Candida albicans,Candida dubliniensis,Candidiasis,DNA 25s,DNA determination,Genotyping,Repetitive Sequence,cost benefit analysis,electrophoresis,fungal strain,gene amplification,genotype,human,molecular typing,mouth cavity,mycosis,nonhuman,oropharynx candidiasis,phylogeny,polymerase chain reaction,repetitive DNA,ribosome DNA,unclassified drug
Subjects: QX Parasitology > QX 500-675 Insects. Other Parasites
Divisions: Education Vice-Chancellor Department > Faculty of Medicine > Department of Basic Science > Department of Parasitology and Mycology
Page Range: pp. 1-6
Journal or Publication Title: Jundishapur Journal of Microbiology
Abstract and Indexing: ISI, Scopus, Other
Quartile : Q4
Volume: 13
Number: 5
Publisher: Kowsar Medical Publishing Company
Identification Number:
ISSN: 20083645 (ISSN)
Depositing User: خانم فائزه مظفری

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