Repository of Research and Investigative Information

Repository of Research and Investigative Information

دانشگاه علوم پزشکی و خدمات بهداشتی درمانی زنجان

Prenatal stress increased gamma 2 GABAA receptor subunit gene expression in hippocampus and potentiated pentylenetetrazol-induced seizure in rats

(2019) Prenatal stress increased gamma 2 GABAA receptor subunit gene expression in hippocampus and potentiated pentylenetetrazol-induced seizure in rats. Iranian Journal of Basic Medical Sciences. pp. 724-729. ISSN 2008-3866, 2008-3874

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Official URL: http://ijbms.mums.ac.ir/article_15208_23707ebd7ad9...

Abstract

Objective(s): Stress during pregnancy is able to bring extensive effects on neurobehavioral development in offspring. The GABAergic system plays a pivotal role in neuronal excitability, which can be affected by prenatal stress (PS). This study aimed to evaluate impact of the PS on gamma 2 subunit of gamma-aminobutyric acid A (GABAA) receptor gene expression in the hippocampus and seizure induced by pentylenetetrazol (PTZ) in developing rats. Materials and Methods: In this experimental study, female Wistar rats were exposed to restraint stress during gestation and their offspring were studied on postnatal days 14 and 21 (P14 and P21, respectively) for epileptic behaviors and gamma 2 GABAA receptor subunit gene expression. Quantitative real-time PCR was used for evaluating the gamma 2 GABAA receptor subunit gene expression in rat pups. Meanwhile, PTZ was injected into the pups, and seizure behaviors were recorded for 60 min. Results: The results showed that gamma 2 subunit mRNA expression significantly increased in the hippocampus of the stressed pups. The expression level of gamma 2 subunit was higher on P21 compared to that on P14 in both groups. Number of seizures with tonic-clonic features increased in pups of stressed group compared to the control group. Prenatal stress significantly caused an increase in the total score of seizure on P21. Conclusion: The effect of PS on seizure susceptibility is age-specific; the increased gamma 2 subunit level in the hippocampus might be, at least in part, the underlying mechanism for PS-induced augmentation of seizures in immature rats.

Item Type: Article
Keywords: GABAA receptor,Infant,Pharmacology & Pharmacy,Prenatal stress,Rat,Research & Experimental Medicine,Seizure,alcohol,allopregnanolone,behaviors,exposure,infant,morphine,restraint stress
Subjects: QU Biochemistry. Cell Biology and Genetics > QU 135-144 Enzymes
Divisions: Education Vice-Chancellor Department > Faculty of Medicine > Department of Basic Science > Department of Molecular Medicine and Genetics
Page Range: pp. 724-729
Journal or Publication Title: Iranian Journal of Basic Medical Sciences
Abstract and Indexing: ISI, Pubmed, Scopus, Other
Volume: 23
Number: 6
Publisher: Mashhad University of Medical Sciences
Identification Number: https://doi.org/10.22038/ijbms.2020.39519.9371
ISSN: 2008-3866, 2008-3874
ISBN: 2008-3866
Depositing User: خانم فائزه مظفری
URI: http://repository.zums.ac.ir/id/eprint/6802

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