Repository of Research and Investigative Information

Repository of Research and Investigative Information

دانشگاه علوم پزشکی و خدمات بهداشتی درمانی زنجان

Simultaneous comparison of L-NAME and melatonin effects on RAW 264.7 cell line's iNOS production and activity

(2019) Simultaneous comparison of L-NAME and melatonin effects on RAW 264.7 cell line's iNOS production and activity. Turkish Journal of Biochemistry. pp. 840-847. ISSN 0250-4685

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Official URL: https://www.scopus.com/inward/record.uri?eid=2-s2....

Abstract

Background: NO (nitric oxide) inhibition could be used for evaluating the drug's efficacy for NO-mediated inflammatory disorders. The aim of this study was to investigate the influence of L-NAME and melatonin on different NO production levels in RAW 264.7 cell line as an in vitro model for inflammatory diseases. Materials and methods: RAW 264.7 macrophage cell line was used to compare the effects of L-NAME and melatonin on basal and Lipopolysaccharide (LPS)-induced iNOS levels. The cells were treated using L-NAME and melatonin for 1 h, afterward incubated with/without LPS for 8 and 24 h. Finally, iNOS mRNA, protein, activity, and nitrite concentrations were evaluated. Results: Inhibition rate of nitrite by 1 mM L-NAME compared with LPS control were 78% and 80% during 8 and 24 h, respectively. Real-time PCR showed that in the LPStreated group, 1 mM L-NAME could result in 14% increase of iNOS-mRNA compared with the control group during 8 h. Dose-dependent activity of iNOS in LPS-induced cells from non-treated to 4 mM L-NAME showed 79% reduction while at the same concentrations of melatonin this decrease was 32% (p-value \textless0.05). Conclusion: L-NAME showed lower iNOS expression modulating efficacy than melatonin. The result concluded lower potential of the NOS synthetic inhibitors rather than melatonin in the treatment of NO-related disorders. © 2019 De Gruyter. All rights reserved.

Item Type: Article
Keywords: Inflammation,L-NAME,Melatonin,Nitric oxide
Divisions: Education Vice-Chancellor Department > Faculty of Medicine > Department of Basic Science > Department of Clinical Biochemistry
Page Range: pp. 840-847
Journal or Publication Title: Turkish Journal of Biochemistry
Abstract and Indexing: ISI, Pubmed, Scopus
Quartile : Q4
Volume: 44
Number: 6
Publisher: DE Gruyter
Identification Number: https://doi.org/10.1515/tjb-2019-0209
ISSN: 0250-4685
Depositing User: خانم فائزه مظفری
URI: http://repository.zums.ac.ir/id/eprint/5739

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