Repository of Research and Investigative Information

Repository of Research and Investigative Information

دانشگاه علوم پزشکی و خدمات بهداشتی درمانی زنجان

Functionalization of anti-Brucella antibody based on SNP and MNP nanoparticles for visual and spectrophotometric detection of Brucella

(2019) Functionalization of anti-Brucella antibody based on SNP and MNP nanoparticles for visual and spectrophotometric detection of Brucella. Spectrochim Acta A Mol Biomol Spectrosc. p. 117891. ISSN 1386-1425, 1873-3557

Full text not available from this repository.

Official URL: https://www.ncbi.nlm.nih.gov/pubmed/31818642

Abstract

An Immuno-Nano-Biosensor with high sensitivity was designed based on iron and silica nanoparticles to detect B. abortus. Briefly explain, primary polyclonal antibody (IgG1) was conjugated on surface magnetic nanoparticles (MNPs) to form MNP-IgG1. Secondary polyclonal antibody (IgG2) and Horseradish Peroxidase enzyme were conjugated on silica nanoparticles (SNPs) to form HRP-SNP-IgG2. HRP-SNP-IgG2. MNP-IgG1 and HRP-SNP-IgG2 were added to B. abortus. The MNP-IgG1-B.abortus-IgG2-SNP-HRP complex was isolated from the reaction mixture using a magnet. After that, tetramethylbenzidine was added to the complex. The reaction was stopped with HCl and investigated using UV-Vis spectrophotometry. The nanoparticles' structure and size were investigated using SEM and DLS. Immuno-Nano-Biosensor sensitivity and specificity were determined. The SEM and DLS results indicated that the SNPs, MNPs, HRP-SNP-IgG2 and MNP-IgG1 size and structure were 35, 44, 60 and 56 nm, respectively. In addition, a good linear correlation was observed at 10(2)-10(7) CFU mL(-)(1) concentrations, which their linear equation and regression were Y = 0.3x + 0.18 and R(2) 0.982, respectively. The limitation of detecting B. abortus was 160 CFU mL(-)(1). Finally, the results demonstrated that those designed Immuno-Nano-Biosensor could be specifically detected B. abortus and B. melitensis in real samples.

Item Type: Article
Keywords: B. abortus,Detection,Immuno-nano-biosensor,MNPs,SNPs
Subjects: QU Biochemistry. Cell Biology and Genetics > QU 100-133 Biochemistry of the Human Body
WQ Obstetrics > WQ 215-270 Pregnancy Complications
Divisions: Education Vice-Chancellor Department > Faculty of Medicine > Department of Basic Science > Department of Clinical Biochemistry
Page Range: p. 117891
Journal or Publication Title: Spectrochim Acta A Mol Biomol Spectrosc
Abstract and Indexing: ISI, Pubmed, Scopus
Quartile : Q1
Volume: 229
Publisher: Elsevier
Identification Number: https://doi.org/10.1016/j.saa.2019.117891
ISSN: 1386-1425, 1873-3557
ISBN: 1386-1425
Depositing User: خانم فائزه مظفری
URI: http://repository.zums.ac.ir/id/eprint/5733

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