Repository of Research and Investigative Information

Repository of Research and Investigative Information

دانشگاه علوم پزشکی و خدمات بهداشتی درمانی زنجان

In vitro regeneration and secondary metabolites of viola caspia subsp. Sylvestrioides marcussen

(2019) In vitro regeneration and secondary metabolites of viola caspia subsp. Sylvestrioides marcussen. Biotechnologia. pp. 407-415. ISSN 0860-7796, 2353-9461

Full text not available from this repository.

Official URL: http://www.biotechnologia-journal.org/In-vitro-reg...

Abstract

Viola caspia subsp. sylvestrioides, a small important medicinal herb, belongs to the subsection Rostratae (sec. Viola) of genus Viola L. This study was performed to develop an efficient protocol for in vitro regeneration of V. caspia subsp. sylvestrioides as an ex situ technique for conservation of endangered plants and extraction of secondary metabolites. Leaf and petiole explants were cultured on half and full-strength Murashige and Skoog (MS) media supplemented with different concentrations and combinations of 6-benzylaminopurine (BAP), isopentenyl adenine (2ip), thidiazuron (TDZ), and naphthalene acetic acid (NAA). Direct organogenesis was induced on half and full-strength MS media supplemented with TDZ (0.7 to 3.5 mg/l) from leaf and petiole explants. Indirect shoot organogenesis was induced on half-strength MS supplemented with 3.5 mg/l 2ip and 2.5 mg/l NAA followed by transferring the obtained callus onto a half-strength MS containing 0.5 mg/l BAP. The highest frequency of shoot organogenesis (100 and 86.66%) was noted for petiole and leaf explants, respectively, on halfstrength MS medium supplemented with 2.8 mg/l TDZ (approx. 7.66 and 4.33 shoots per petiole and leaf explant, respectively). The induced shoots were used for root induction on a half-strength MS medium with 0.5 mg/l indole-3-butyric acid (IBA). Phytochemical constituents in leaf tincture were determined by gas chromatography/mass spectrometry (GC/MS) analysis. GC/MS results revealed that vitamin E was the main component in the tincture of Viola leaves. The present study provides a simple and rapid protocol for in vitro regeneration of V. caspia subsp. sylvestrioides plants which can be used for gene transfer and conservation purposes, and in pharmaceutical studies. © 2019, Institute of Bioorganic Chemistry. All rights reserved.

Item Type: Article
Keywords: GC/MS analysis,Organogenesis,Thidiazuron,Viola,Vitamin E
Divisions: vice research > زیست فناوری دارویی
Page Range: pp. 407-415
Journal or Publication Title: Biotechnologia
Abstract and Indexing: Scopus
Quartile : Q4
Volume: 100
Number: 4
Identification Number: https://doi.org/10.5114/bta.2019.90241
ISSN: 0860-7796, 2353-9461
Depositing User: خانم فائزه مظفری
URI: http://repository.zums.ac.ir/id/eprint/5710

Actions (login required)

View Item View Item