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Repository of Research and Investigative Information

دانشگاه علوم پزشکی و خدمات بهداشتی درمانی زنجان

Virulence characteristics of multidrug resistant biofilm forming Acinetobacter baumannii isolated from intensive care unit patients

(2019) Virulence characteristics of multidrug resistant biofilm forming Acinetobacter baumannii isolated from intensive care unit patients. BMC Infect Dis. p. 629. ISSN 1471-2334

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Official URL: https://static-content.springer.com/pdf/art%3A10.1...

Abstract

BACKGROUND: Nosocomial infections and persistence of multidrug resistant biofilm forming Acinetobacter baumannii in hospitals has made it as a serious problem in healthcare settings worldwide. METHODS: A total of 100 A. baumannii clinical isolates from immunocompromised patients hospitalized in ICU were investigated for biofilm formation, the presence of biofilm related genes (bap, ompA, csuE, fimH, epsA, blaPER-1, bfmS, ptk, pgaB, csgA, kpsMII), integron characterization and molecular typing based on REP-PCR. RESULTS: All isolates were resistant to three or more categories of antibiotics and considered as multidrug resistant (MDR). A total of 32 isolates were resistant to all tested antibiotics and 91% were extensively drug-resistance (XDR). All isolates were able to produce biofilm and 58% of isolates showed strong ability to biofilm formation. All strong biofilm forming A. baumannii isolates were XDR. All A. baumannii isolates carried at least one biofilm related gene. The most prevalent gene was csuE (100%), followed by pgaB (98%), epsA and ptk (95%), bfmS (92%) and ompA (81%). 98% of isolates carried more than 4 biofilm related genes, simultaneously. Class I integron (67%) was more frequent in comparison with class II (10%) (P \textless 0.05). The REP-PCR patterns were classified as 8 types (A-H) and 21 subtypes. The A1 (23%) and C1 (15%) clusters were the most prevalent among A. baumannii isolates (P \textless 0.05). According to the REP-PCR patterns, 23% of all isolates had a clonal relatedness. CONCLUSION: Our study revealed the high frequency of biofilm forming XDR A. baumannii in ICU patients, with a high prevalence of biofilm related genes of csuE and pgaB. It seems that the appropriate surveillance and control measures are essential to prevent the emergence and transmission of XDR A. baumannii in our country.

Item Type: Article
Keywords: Acinetobacter Infections/microbiology,Acinetobacter baumannii,Acinetobacter baumannii/*drug effects/genetics/isolation &,Anti-Bacterial Agents/pharmacology,Biofilm,Biofilms/drug effects,Cross Infection/microbiology,Drug Resistance, Multiple, Bacterial/drug effects/*genetics,Humans,Immunocompromised Host,Integron,Intensive Care Units,Iran,Microbial Sensitivity Tests,Polymerase Chain Reaction,Rep-pcr,Virulence,Virulence factor,purification/*pathogenicity
Subjects: QW Microbiology and Immunology > QW 1-300 Microbiology
Divisions: Education Vice-Chancellor Department > Faculty of Medicine > Department of Basic Science > Department of Microbiology
Page Range: p. 629
Journal or Publication Title: BMC Infect Dis
Abstract and Indexing: ISI, Pubmed, Scopus
Quartile : Q3
Volume: 19
Number: 1
Publisher: ProQuest
Identification Number: https://doi.org/10.1186/s12879-019-4272-0
ISSN: 1471-2334
ISBN: 1471-2334
Depositing User: خانم فائزه مظفری
URI: http://repository.zums.ac.ir/id/eprint/5591

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