Repository of Research and Investigative Information

Repository of Research and Investigative Information

دانشگاه علوم پزشکی و خدمات بهداشتی درمانی زنجان

High incidence of virulence determinants, aminoglycoside and vancomycin resistance in enterococci isolated from hospitalized patients in Northwest Iran

(2019) High incidence of virulence determinants, aminoglycoside and vancomycin resistance in enterococci isolated from hospitalized patients in Northwest Iran. BMC Infect Dis. p. 744. ISSN 1471-2334

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Official URL: https://pubmed.ncbi.nlm.nih.gov/31455296/

Abstract

BACKGROUND: Multidrug resistant (MDR) enterococci are important nosocomial pathogens causing serious problem in hospitalized patients. The aim of present study was to investigate the frequency of high-level aminoglycoside-resistant and vancomycin-resistant enterococci (VRE) and virulence encoding genes in enterococci isolated from hospitalized patients. METHODS: A total of 100 enterococci isolated from urine samples of hospitalized patients with symptomatic urinary tract infections were investigated for antimicrobial susceptibility, the frequency of aminoglycoside and vancomycin resistance genes (including aac (6')-Ie-aph (2")-Ia, aph (3')-IIIa, ant (4')-Ia, aph (2")-Ic, aph (2")-Ib, aph (2")-Id, ant (3'')-III, ant (6')-Ia, vanA, vanB and vanC) and virulence encoding genes (including gelE, PAI, esp, ace, cyl, hyl and sprE). RESULTS: Enterococcus faecalis species was identified as predominant enterococci (69%), followed by "other" Enterococcus species (21%) and E. faecium (10%). Ninety three percent of isolates were resistant to one or more antimicrobial agents, with the most frequent resistance found against tetracycline (86%), ciprofloxacin (73%) and quinupristin-dalfopristin (53%). Gentamicin and streptomycin resistance were detected in 50 and 34% of isolates, respectively. The most prevalent aminoglycoside resistance genes were ant (3'')-III (78%) and aph (3')-IIIa (67%). Vancomycin resistance was detected in 21% of isolates. All E. faecium isolates carried vanA gene, whereas, the vanB gene was not detected in Enterococcus species. The most frequent virulence gene was ace (88.6%), followed by esp (67.1%), PAI (45.5%) and sprE (41.7%). CONCLUSION: Our study revealed the high frequency of gentamycin resistance and VRE in E. faecium isolates, with a high prevalence and heterogeneity of virulence and resistance genes. Due to high frequency of MDR enterococci, it seems that the appropriate surveillance and control measures are essential to prevent the emergence and transmission of these isolates in hospitals.

Item Type: Article
Keywords: Adult,Aminoglycoside resistance,Aminoglycosides/pharmacology,Anti-Bacterial Agents/pharmacology,Drug Resistance, Bacterial/drug effects/*genetics,Enterococci,Enterococcus/drug effects/genetics/*pathogenicity,Gentamicins/pharmacology,Gram-Positive Bacterial Infections/drug therapy/epidemiology/*microbiology,Hospitalization,Humans,Incidence,Iran,Microbial Sensitivity Tests,Middle Aged,Urinary Tract Infections/drug therapy/epidemiology/*microbiology,Vancomycin Resistance/drug effects/genetics,Vancomycin resistance,Vancomycin-Resistant Enterococci/drug effects/pathogenicity,Virulence Factors/*genetics,Virulence factors
Subjects: QW Microbiology and Immunology > QW 1-300 Microbiology
Divisions: Education Vice-Chancellor Department > Faculty of Medicine > Department of Basic Science > Department of Microbiology
Page Range: p. 744
Journal or Publication Title: BMC Infect Dis
Abstract and Indexing: ISI, Pubmed, Scopus
Quartile : Q3
Volume: 19
Number: 1
Publisher: ProQuest
Identification Number: https://doi.org/10.1186/s12879-019-4395-3
ISSN: 1471-2334
ISBN: 1471-2334
Depositing User: خانم فائزه مظفری
URI: http://repository.zums.ac.ir/id/eprint/5489

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