Repository of Research and Investigative Information

Repository of Research and Investigative Information

دانشگاه علوم پزشکی و خدمات بهداشتی درمانی زنجان

Genetic Analysis of Congenital Heart Disease in Iranian Pediatric Patients

(2019) Genetic Analysis of Congenital Heart Disease in Iranian Pediatric Patients. International Cardiovascular Research Journal. pp. 56-61. ISSN 2251-9130

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Background: Congenital Heart Disease (CHD) occurs in nearly 1% of newborns due to genetic and environmental factors. There are many genes involved in CHD. Variants of Gap Junction Protein Alpha 1 (GJA1), Zic Family Member 3 (ZIC3), Nodal Growth Differentiation Factor (NODAL), and Forkhead Box H1 (FOXH1) genes are common to develop CHD. Objectives: To date, no study has been published about CHD patients in Iran. Therefore, the present study aimed to evaluate the sequence variants of these genes in Iranian patients. Methods: This study was conducted on 73 patients with familial CHD and their family members. Genetic investigations were performed using Polymerase Chain Reaction (PCR) and direct DNA sequencing of the exons and flanking regions of the genes. The variants were evaluated using available online software tools. Mutation taster, PROVEAN, SIFT, PolyPhen-2, and CADD were used to predict the effects of the variants and I-TASSER was applied to evaluate the possible structural effects of the genetic variations. Results: c.612G \textgreater A, c.717G \textgreater A, and c.895C \textgreater T in GJA1 were found in the study participants. c.1248T \textgreater G in the ZIC3 was observed in a twin with CHD. Besides, c.193 + 12C \textgreater T, c.-109T \textgreater C, c.494A \textgreater G, c.417C \textgreater T, and c.357C \textgreater T variants were detected in the NODAL gene. Additionally, c.-314T \textgreater G, c.175-30C \textgreater T, and c.373A \textgreater T sequence changes were determined in the FOXH1 gene. Two novel heterozygous variants, namely c.1061C \textgreater G and c.-465C \textgreater A, were also found in the FOXH1 gene. Bioinformatics analysis indicated that the detected reported/novel variants might not have a damaging effect among Iranian CHD patients. Conclusion: The study results indicated the first variation screening of GJA1, ZIC3, NODAL, and FOXH1 genes in Iranian familial CHD patients. The results also suggested a minor role for GJA1, ZIC3, NODAL, and FOXH1 genes in familial CHD pathogenesis. However, their exact role in CHD causation entails further research.

Item Type: Article
Keywords: Mutation Congenital Heart Disease Etiology
Subjects: Cardiovascular System > WG 200-460 Heart. Heart Diseases
Divisions: Education Vice-Chancellor Department > Faculty of Medicine > Department of Basic Science > Department of Molecular Medicine and Genetics
Page Range: pp. 56-61
Journal or Publication Title: International Cardiovascular Research Journal
Abstract and Indexing: ISI, Scopus
Volume: 13
Number: 2
ISSN: 2251-9130
ISBN: 2251-9130
Depositing User: خانم فائزه مظفری

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