Repository of Research and Investigative Information

Repository of Research and Investigative Information

دانشگاه علوم پزشکی و خدمات بهداشتی درمانی زنجان

Cyanidin 3-O-glucoside Induces Apoptosis on Osteosarcoma Cells through Upregulation of PPARgamma and P21: An In Vitro Study

(2020) Cyanidin 3-O-glucoside Induces Apoptosis on Osteosarcoma Cells through Upregulation of PPARgamma and P21: An In Vitro Study. Anticancer Agents Med Chem. ISSN 1871-5206

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Official URL: https://pubmed.ncbi.nlm.nih.gov/32268872/

Abstract

BACKGROUND: Osteosarcoma is known as the malignant tumors of bone. Cyanidin 3-O-glucoside (C3G) has a powerful ability to induce apoptotic cell death in the different cancer cells however the mechanisms of action for C3G have not been clarified yet. OBJECTIVE: In this study, we have investigated the apoptotic effects of C3G on three different osteosarcoma cell lines including Saso-2, MG-63, G-292 (clone A141B1). METHODS: The 24 hours IC50 of C3G for Saso-2, G-292, and MG-63 cells was evaluated by MTT assay. Apoptosis induction in these cell lines after treatment with C3G approved by the way of Annexin V/PI flow cytometry. Changes at the mRNA expression level of PPARgamma, P21, Bax, and Bcl-xl genes have investigated by real-time PCR technique, as well as P21 expression was further confirmed by western blotting. RESULTS: The MTT assay results have demonstrated that the 24 hours IC50 of C3G for Saso-2 and G-292 cells was 110mug/ml while it was about 140mug/ml for the MG-63 cells. The results of real-time PCR have clearly shown that treatment of the cells with 24 hours IC50 of C3G case to upregulation of PPARgamma, P21, and Bax genes. Also, western blot analyzing confirmed that P21 protein overexpressed endogenously after treatment of cells with C3G which it was more upregulated in MG-63 cells compared to the other cell lines. CONCLUSION: The final outcomes introduce the C3G as a novel anti-osteosarcoma agent with the ability to induce apoptosis in different osteosarcoma cells via upregulation of the PPARgamma and the P21.

Item Type: Article
Keywords: Cyanidin 3-O-glucoside,Mtt.,Osteosarcoma,P21,PPARgamma,apoptosis
Subjects: WE Musculoskeletal System > WE 200-259 Bones
Divisions: Education Vice-Chancellor Department > Faculty of Pharmacy > Department of Clinical Pharmacy
Journal or Publication Title: Anticancer Agents Med Chem
Abstract and Indexing: ISI, Pubmed, Scopus
Quartile : Q3
Publisher: Bentham
Identification Number: https://doi.org/10.2174/1871520620666200408081111
ISSN: 1871-5206
ISBN: 1871-5206
Depositing User: خانم فائزه مظفری
URI: http://repository.zums.ac.ir/id/eprint/5464

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