Repository of Research and Investigative Information

Repository of Research and Investigative Information

دانشگاه علوم پزشکی و خدمات بهداشتی درمانی زنجان

In vitro differentiation of conjunctiva mesenchymal stem cells into insulin producing cells on natural and synthetic electrospun scaffolds

(2019) In vitro differentiation of conjunctiva mesenchymal stem cells into insulin producing cells on natural and synthetic electrospun scaffolds. Biologicals. pp. 33-38. ISSN 1045-1056

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Official URL: https://www.sciencedirect.com/science/article/pii/...

Abstract

Polymers are used in tissue engineering as a scaffold. In this study the differentiation capability of conjunctiva mesenchymal stem cells (CJMSCs) on natural and synthetic nanofibrous electrospun scaffolds into insulin producing cells (IPCs) were studied. Natural Silk fibroin and synthetic PLLA polymers were used to fabricate electrospun scaffolds. These scaffolds are characterized by SEM and CJMSCs were differentiated into IPCs on these scaffolds. The differentiation efficiency was measured by analysis the expression of specific pancreatic markers by RT-qPCR and insulin release capacity via ELISA. Microscopy analysis showed the fabrication of uniform nanofibers and the formation of the islet-like clusters at the end of differentiation period. Significant differences in expression of Pdx-1 and glucagon were observed in PLLA scaffold compared to Silk scaffold (Fold: 1.625 and 1.434, respectively; P-value\textless/=0.0001 for both). Furthermore, insulin secretion at high glucose concentration was significantly higher in cells differentiated on PLLA scaffold than those cultured on Silk scaffold (P-value: 0.012). The scaffolds can enhance the differentiation of IPCs from CJMSCs. In this way, PLLA synthetic scaffold was more efficient than Silk natural scaffold. We conclude that the nano fi brous scaffolds reported herein could be used as a potential supportive matrix for islet tissue engineering.

Item Type: Article
Keywords: *Cell Differentiation,Cells, Cultured,Conjunctiva/cytology/*metabolism,Electrospun scaffolds,Fibroins/*chemistry,Humans,Insulin-Secreting Cells/cytology/*metabolism,Insulin-producing cells,Mesenchymal Stem Cells/cytology/*metabolism,Nanofibers/*chemistry,Poly(L-lactic) acid,Silk fibroin,Tissue Scaffolds/*chemistry
Subjects: QU Biochemistry. Cell Biology and Genetics > QU 300-560 Cell Biology and Genetics
Page Range: pp. 33-38
Journal or Publication Title: Biologicals
Abstract and Indexing: ISI, Pubmed, Scopus
Quartile : Q3
Volume: 62
Publisher: Elsevier
Identification Number: https://doi.org/10.1016/j.biologicals.2019.10.004
ISSN: 1045-1056
ISBN: 1045-1056
Depositing User: خانم فائزه مظفری
URI: http://repository.zums.ac.ir/id/eprint/5441

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