Repository of Research and Investigative Information

Repository of Research and Investigative Information

دانشگاه علوم پزشکی و خدمات بهداشتی درمانی زنجان

A hybrid microfluidic system for regulation of neural differentiation in induced pluripotent stem cells

(2016) A hybrid microfluidic system for regulation of neural differentiation in induced pluripotent stem cells. Journal of Biomedical Materials Research Part A. pp. 1534-1543. ISSN 1549-3296

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Abstract

Controlling cellular orientation, proliferation, and differentiation is valuable in designing organ replacements and directing tissue regeneration. In the present study, we developed a hybrid microfluidic system to produce a dynamic microenvironment by placing aligned PDMS microgrooves on surface of biodegradable polymers as physical guidance cues for controlling the neural differentiation of human induced pluripotent stem cells (hiPSCs). The neuronal differentiation capacity of cultured hiPSCs in the microfluidic system and other control groups was investigated using quantitative real time PCR (qPCR) and immunocytochemistry. The functionally of differentiated hiPSCs inside hybrid system's scaffolds was also evaluated on the rat hemisected spinal cord in acute phase. Implanted cell's fate was examined using tissue freeze section and the functional recovery was evaluated according to the Basso, Beattie, and Bresnahan (BBB) locomotor rating scale. Our results confirmed the differentiation of hiPSCs to neuronal cells on the microfluidic device where the expression of neuronal-specific genes was significantly higher compared to those cultured on the other systems such as plain tissue culture dishes and scaffolds without fluidic channels. Although survival and integration of implanted hiPSCs did not lead to a significant functional recovery, we believe that combination of fluidic channels with nanofiber scaffolds provides a great microenvironment for neural tissue engineering, and can be used as a powerful tool for in situ monitoring of differentiation potential of various kinds of stem cells. (c) 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 1534-1543, 2016.

Item Type: Article
Keywords: microfluidics human induced pluripotent stem cells (hiPSCs) neural differentiation tissue engineering nanofibers SPINAL-CORD-INJURY FUNCTIONAL RECOVERY PROGENITOR CELLS DEFINED FACTORS IPS CELLS NEURONS FIBROBLASTS SCAFFOLDS DISEASE REPAIR Engineering, Biomedical Materials Science, Biomaterials
Page Range: pp. 1534-1543
Journal or Publication Title: Journal of Biomedical Materials Research Part A
Abstract and Indexing: ISI, Pubmed, Scopus
Quartile : Q2
Volume: 104
Number: 6
Identification Number: https://doi.org/10.1002/jbm.a.35689
ISSN: 1549-3296
Depositing User: خانم مریم زرقانی
URI: http://repository.zums.ac.ir/id/eprint/532

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