Repository of Research and Investigative Information

Repository of Research and Investigative Information

دانشگاه علوم پزشکی و خدمات بهداشتی درمانی زنجان

Development of a loop-mediated isothermal amplification assay for rapid detection of Burkholderia mallei

(2016) Development of a loop-mediated isothermal amplification assay for rapid detection of Burkholderia mallei. Cellular and Molecular Biology. pp. 32-36. ISSN 0145-5680

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Abstract

The present study was conducted to establish a Loop-mediated isothermal amplification (LAMP) technique for the rapid detection of B. mallei the etiologic agent of glanders, a highly contagious disease of equines. A set of six specific primers targeting integrase gene cluster were designed for the LAMP test. The reaction was optimized using different temperatures and time intervals. The specificity of the assay was evaluated using DNA from B. pseudomallei and Pseudomonas aeruginosa. The LAMP products were analyzed both visually and under UV light after electrophoresis. The optimized conditions were found to be at 63 degrees C for 60 min. The assay showed high specificity and sensitivity. It was concluded that the established LAMP assay is a rapid, sensitive and practical tool for detection of B. mallei and early diagnosis of glanders.

Item Type: Article
Keywords: Glanders identification Burkholderia mallei LAMP LAMP ASSAY PSEUDOMALLEI PCR GLANDERS VIRUS IDENTIFICATION ESTABLISHMENT THAILANDENSIS DIAGNOSIS SYSTEM Biochemistry & Molecular Biology Cell Biology
Page Range: pp. 32-36
Journal or Publication Title: Cellular and Molecular Biology
Abstract and Indexing: ISI, Scopus
Quartile : Q4
Volume: 62
Number: 10
Identification Number: https://doi.org/10.14715/cmb/2016.62.10.5
ISSN: 0145-5680
Depositing User: خانم مریم زرقانی
URI: http://repository.zums.ac.ir/id/eprint/504

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