Repository of Research and Investigative Information

Repository of Research and Investigative Information

دانشگاه علوم پزشکی و خدمات بهداشتی درمانی زنجان

Ameliorating Effect of Ginseng on Epididymo-Orchitis Inducing Alterations in Sperm Quality and Spermatogenic Cells Apoptosis following Infection by Uropathogenic Escherichia coli in Rats

(2016) Ameliorating Effect of Ginseng on Epididymo-Orchitis Inducing Alterations in Sperm Quality and Spermatogenic Cells Apoptosis following Infection by Uropathogenic Escherichia coli in Rats. Cell Journal. pp. 446-457. ISSN 2228-5806

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Abstract

Objective: Epididymo-orchitis (EO) potentially results in reduced fertility in up to 60 of affected patients. The anti-inflammatory effects of Korean red ginseng (KRG) and its ability to act as an immunoenhancer in parallel with the beneficial effects of this ancient herbal medicine on the reproductive systems of animals and humans led us to evaluate its protective effects against acute EO. Materials and Methods: This animal experimental study was conducted in the Department of Anatomical Sciences, Faculty of Medicine, Zanjan University of Medical Sciences (ZUMS), Zanjan, Iran during 2013-2015. We divided 50 Wistar rats into five following groups (n= 10 per group): i. Control-intact animals, ii. Vehicle-phosphate buffered saline (PBS) injection into the vas deferens, iii. KRG-an intraperitoneal (IP) injection of KRG, iv. EO-an injection of uropathogenic Escherichia coli (UPEC) strain M39 into the vas deferens, and v. EO/KRG-injections of both UPEC strain M39 and KRG. The treatment lasted seven days. We then evaluated sperm parameters, number of germ cell layers, Johnson's criteria, germ cell apoptosis, body weight and relative sex organs weight. Results: Acute EO increased the relative weight of prostate and seminal vesicles (P=0.05). It also reduced sperm quality such as total motility, sperm concentration (P=0.01), and the percentage of normal sperm (P=0.001). Moreover, acute EO decreased Miller's (P=0.05) and Johnsen's scores and increased apoptotic indexes of spermatogenic cells (P=0.001). KRG treatment decreased prostate weight gain (P=0.05) and improved the percentage of sperm with normal morphology, total motility (P=0.01), and progressive motility (P=0.05). The apoptotic indexes of spermatogenic cells reduced (P=0.001), whereas both Johnsen's (P=0.01) and Miller's criteria increased in the KRG-treated EO testis (P=0.05). Conclusion: Consequently, KRG ameliorated the devastating effects of EO on the sperm retrieved from either epididymis or testicle in rats.

Item Type: Article
Keywords: Ginseng Uropathogenic Escherichia coli Sperm Rat Testis KOREAN RED GINSENG PANAX-GINSENG NITRIC-OXIDE IN-VITRO ERECTILE DYSFUNCTION TESTICULAR FUNCTION IMMUNE PRIVILEGE TRACT-INFECTION MALE-FERTILITY EXTRACT G115 Cell Biology
Page Range: pp. 446-457
Journal or Publication Title: Cell Journal
Abstract and Indexing: ISI, Pubmed, Scopus
Quartile : Q3
Volume: 18
Number: 3
Identification Number: https://www.ncbi.nlm.nih.gov/pubmed?term=Ameliorating%20Effect%20of%20Ginseng%20on%20Epididymo-Orchitis%5BTitle%5D
ISSN: 2228-5806
Depositing User: خانم مریم زرقانی
URI: http://repository.zums.ac.ir/id/eprint/496

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