Repository of Research and Investigative Information

Repository of Research and Investigative Information

دانشگاه علوم پزشکی و خدمات بهداشتی درمانی زنجان

Detection of extended-spectrum beta-lactamase (ESBL) and plasmid-borne bla(CTX-M) and bla(TEM) genes among clinical strains of Escherichia coli isolated from patients in the north of Iran

(2016) Detection of extended-spectrum beta-lactamase (ESBL) and plasmid-borne bla(CTX-M) and bla(TEM) genes among clinical strains of Escherichia coli isolated from patients in the north of Iran. Journal of Global Antimicrobial Resistance. pp. 110-113. ISSN 2213-7165

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Official URL: <Go to ISI>://WOS:000391780900025

Abstract

Escherichia coli is an important cause of hospital-acquired infections worldwide. Antimicrobial resistance leads to treatment failure of hospital infections caused by E. coli. Production of extended-spectrum P-lactamases (ESBLs) is one of the major causes of antibiotic resistance in these bacteria. This study aimed to investigate the frequency of bla(TEM) and bla(CTX-M) genes in ESBL-producing E. coli strains isolated from clinical specimens of patients admitted to six hospitals in the north of Iran. A total of 160 E. coli strains were isolated from various clinical samples of hospitalised patients. Antibiotic resistance patterns were determined by the Kirby-Bauer disk diffusion method. The double-disk phenotypic confirmatory test was carried out amongst p-lactam-resistant isolates to detect ESBL-producing strains. Plasmid DNA of ESBL-producing strains was extracted and subjected to PCR for detection of the bla(TEM) and bla(CTX-M) genes, and isolates were extensively verified by sequencing. The highest resistance rate was to amoxicillin; all E. coli isolates (100) were susceptible to imipenem. Amongst the 160 clinical E. coli isolates, 83 (51.9) were ESBL-positive, of which 27 (32.5) and 72 (86.7) were positive for bla(TEM) and bla(CTX-M), respectively. This study is the first report of an ESBL phenotype disseminated in hospitals in the north of Iran. These findings showed that there was a direct relationship between the development of resistance to beta-lactam antibiotics and production of TEM and CTX-M enzymes. (C) 2016 International Society for Chemotherapy of Infection and Cancer. Published by Elsevier Ltd. All rights reserved.

Item Type: Article
Keywords: Escherichia coli ESBL PCR TEM gene CTX-M gene CTX-M PREVALENCE KLEBSIELLA SHV TEM BAUMANNII EMERGENCE Infectious Diseases Pharmacology & Pharmacy
Page Range: pp. 110-113
Journal or Publication Title: Journal of Global Antimicrobial Resistance
Abstract and Indexing: ISI, Pubmed, Scopus
Quartile : Q3
Volume: 7
Identification Number: https://doi.org/10.1016/j.jgar.2016.08.005
ISSN: 2213-7165
Depositing User: خانم مریم زرقانی
URI: http://repository.zums.ac.ir/id/eprint/458

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