Repository of Research and Investigative Information

Repository of Research and Investigative Information

دانشگاه علوم پزشکی و خدمات بهداشتی درمانی زنجان

Supplementation of freezing media with stromal cell-derived factor-1α preserves human sperm from cryodamage

(2017) Supplementation of freezing media with stromal cell-derived factor-1α preserves human sperm from cryodamage. Cryobiology. pp. 37-42. ISSN 00112240 (ISSN)

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Official URL: https://www.scopus.com/inward/record.uri?eid=2-s2....

Abstract

The destructive effects of sperm cryopreservation result in reduced sperm motility and increased apoptosis. Oocytes, endometrium, and follicular fluid express stromal cell-derived factor-1 alpha (SDF-1α) or C-X-C motif chemokine ligand 12 (CXCL12) while its specific receptor chemokine, CXC motif receptor 4 (CXCR4) is expressed in the head of sperm. SDF-1α can increase sperm motility and preserve normal mitochondrial status. The present study intends to investigate whether the addition of SDF-1α to freezing extender can facilitate cryosurvival of spermatozoa and how SDF-1α protects spermatozoa against damages during cryopreservation. In this study, we collected 22 semen samples from healthy donors and treated them with different concentrations of SDF-1α followed by cryopreservation for one month. We measured sperm motility by CASA, mitochondrial ROS generation by flow cytometry using the probe MitoSOX Red™ (MSR) to measure mitochondrial superoxide anion (O2–•), DNA fragmentation by flow cytometry according to the TUNEL kit, and expressions of Bcl-2 and Bax by RT-qPCR in freeze-thawed sperm. The results showed that SDF-1α attenuated mitochondrial ROS generation at different doses, particularly the 250 ng/ml treated samples which, in turn, reduced the expressions of pro-apoptotic genes such as Bax. Eventually, SDF-1α reduced DNA fragmentation and ameliorated sperm motility in the 1–100 ng/ml treated samples during cryopreservation. The present study, for the first time, demonstrated that SDF-1α dose-dependently moderated oxidative stress injury in human sperm by reduction of mitochondrial ROS generation. It could subsequently cause a decrease in apoptosis during freeze-thawing and protect human spermatozoa from cryodamage. © 2017 Elsevier Inc.

Item Type: Article
Keywords: Apoptosis Chemokine CXCL12 Cryopreservation Reactive oxygen species Sperm protein Bax protein bcl 2 reactive oxygen metabolite stromal cell derived factor 1alpha superoxide cryoprotective agent CXCL12 protein, human stromal cell derived factor 1 Article Bax gene cell damage comparative study concentration (parameters) controlled study cryodamage DNA fragmentation flow cytometry freeze thawing freezing medium human human cell male mitochondrion molecular dynamics normal human oxidative stress priority journal protein expression reverse transcription polymerase chain reaction spermatozoon spermatozoon motility TUNEL assay adult drug effects freezing metabolism procedures sperm preservation bcl-2-Associated X Protein Cryoprotective Agents Humans Mitochondria Proto-Oncogene Proteins c-bcl-2 Semen Preservation Sperm Motility Spermatozoa
Page Range: pp. 37-42
Journal or Publication Title: Cryobiology
Abstract and Indexing: ISI, Pubmed, Scopus
Quartile : Q2
Volume: 79
Identification Number: https://doi.org/10.1016/j.cryobiol.2017.09.004 https://www.ncbi.nlm.nih.gov/pubmed/28947251
ISSN: 00112240 (ISSN)
Depositing User: خانم مریم زرقانی
URI: http://repository.zums.ac.ir/id/eprint/349

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