Repository of Research and Investigative Information

Repository of Research and Investigative Information

دانشگاه علوم پزشکی و خدمات بهداشتی درمانی زنجان

Preliminary study showing no association between G238A (rs361525) tumor necrosis factor-alpha (TNF-alpha) gene polymorphism and its serum level, hormonal and biochemical aspects of polycystic ovary syndrome

(2018) Preliminary study showing no association between G238A (rs361525) tumor necrosis factor-alpha (TNF-alpha) gene polymorphism and its serum level, hormonal and biochemical aspects of polycystic ovary syndrome. BMC Med Genet. p. 149.

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Abstract

BACKGROUND: Polycystic ovary syndrome (PCOS) is the main cause of female infertility. Interactions among genetic, biochemical, and immunological factors can affect the pathogenesis of PCOS. As a proinflammatory cytokine, tumor necrosis factor-alpha (TNF-alpha) plays an important role in this regard. The present study aimed to evaluate the association of the rs361525 gene single-nucleotide polymorphism (SNP) and TNF-alpha serum levels with the hormonal and biochemical characteristics of PCOS in Iranian individuals. METHODS: The SNP rs361525 in the TNF-alpha gene was analyzed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) in a total of 111 PCOS patients and 105 healthy females. Serum levels of TNF-alpha, lipid and hormone profiles, and biochemical factors were measured using enzyme-linked immunosorbent assay (ELISA) and calorimetric methods, as appropriate. RESULTS: The TNF-alpha serum level was higher in women with PCOS compared with the control group (p \textless 0.0001), and it was significantly correlated with the homeostasis model assessment (HOMA) factor (r = 0.138, p \textless 0.05). No significant differences were found in the genotype and allelic frequencies between the two groups (p \textgreater 0.05). Higher levels and significant differences were found for the HOMA factor, luteinizing hormone/follicle-stimulating hormone (LH/FSH), testosterone, and body mass index (BMI) in the PCOS group compared with the control group (p \textless 0.0001). High LH/FSH ratios (odds ratio OR = 1.98, 95{\%} confidence interval CI = 1.20-3.28, p {\textless} 0.01), and high HOMA factor (OR = 5.04, 95{\%} CI = 2.82-9.01, p {\textless} 0.001) were significantly associated with an increased risk of PCOS. CONCLUSIONS: Despite the lack of significant difference between rs361525 polymorphism of the TNF-alpha gene and PCOS, the serum level of TNF-alpha was increased in PCOS patients and positively correlated with the HOMA factor. Elevation of the LH/FSH ratio and HOMA for insulin resistance (HOMA-IR) increased the risk of PCOS. Therefore, TNF-alpha could indirectly contribute to PCOS progression.

Item Type: Article
Keywords: *Hormone profile,*Polycystic ovary syndrome (PCOS),*Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP),*Polymorphism,*Tumor necrosis factor-alpha (TNF-alpha),Adult,Body Mass Index,Case-Control Studies,Female,Gene Frequency/genetics,Genetic Predisposition to Disease/*genetics,Hormones/genetics,Humans,Insulin Resistance/genetics,Iran,Polycystic Ovary Syndrome/*genetics,Polymorphism, Restriction Fragment Length/genetics,Polymorphism, Single Nucleotide/*genetics,Tumor Necrosis Factor-alpha/*blood/*genetics
Page Range: p. 149
Journal or Publication Title: BMC Med Genet
Abstract and Indexing: ISI, Pubmed, Scopus
Quartile : Q3
Volume: 19
Number: 1
Identification Number: https://doi.org/10.1186/s12881-018-0662-1
ISBN: 1471-2350
Depositing User: خانم مریم زرقانی
URI: http://repository.zums.ac.ir/id/eprint/3102

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