Repository of Research and Investigative Information

Repository of Research and Investigative Information

دانشگاه علوم پزشکی و خدمات بهداشتی درمانی زنجان

Methotrexate-conjugated L-lysine coated iron oxide magnetic nanoparticles for inhibition of MCF-7 breast cancer cells

(2018) Methotrexate-conjugated L-lysine coated iron oxide magnetic nanoparticles for inhibition of MCF-7 breast cancer cells. Drug Development and Industrial Pharmacy. pp. 886-894.

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Official URL: https://www2.scopus.com/inward/record.uri?eid=2-s2...

Abstract

Methotrexate (MTX), a stoichiometric inhibitor of dihydrofolate reductase enzyme, is a chemotherapeutic agent for treating a diversity of neoplasms. In this study, we design and developed a new formulation of MTX that serves as drug carrier and examined its cytotoxic effect in vitro. This target drug delivery system is dependent on the release of the MTX within the lysosomal compartment. The iron oxide magnetic nanoparticles (IONPs) were first surface-coated with L-lysine and subsequently conjugated with MTX through amidation between the carboxylic acid end groups on MTX and the amine groups on the IONPs surface. MTX-conjugated L-lysine coated IONPs (F-Lys-MTX NPs) was characterized by X-ray diffraction, thermogravimetric analysis, differential scanning calorimetry, Fourier transform infrared spectroscopy, vibrating sample magnetometer, and transmission electron microscopy techniques. The cytotoxicity of the void of MTX and F-Lys-MTX NPs were compared to each other by MTT assay of the treated MCF-7 cell lines. The results showed that the ζ-potential of F-Lys-MTX NPs was about â��5.49 mV and the average size was 43.72 ± 4.73 nm. Model studies exhibited the release of MTX via peptide bond cleavage in the presence of proteinase K and at low pH. These studies specify that F-Lys-MTX NPs have a very remarkable anticancer effect, for breast cancer cell lines. © 2017 Informa UK Limited, trading as Taylor & Francis Group.

Item Type: Article
Keywords: Drug conjugated; iron oxide; magnetic nanoparticles; L-lysine; drug delivery; methotrexate
Page Range: pp. 886-894
Journal or Publication Title: Drug Development and Industrial Pharmacy
Abstract and Indexing: ISI, Pubmed, Scopus
Quartile : Q3
Volume: 44
Number: 6
Identification Number: https://doi.org/10.1080/03639045.2017.1417422
Depositing User: خانم مریم زرقانی
URI: http://repository.zums.ac.ir/id/eprint/3081

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