Repository of Research and Investigative Information

Repository of Research and Investigative Information

دانشگاه علوم پزشکی و خدمات بهداشتی درمانی زنجان

Evaluating the miR-302b and miR-145 Expression in Formalin-Fixed Paraffin-Embedded Samples of Esophageal Squamous Cell Carcinoma

(2015) Evaluating the miR-302b and miR-145 Expression in Formalin-Fixed Paraffin-Embedded Samples of Esophageal Squamous Cell Carcinoma. ARCHIVES OF IRANIAN MEDICINE. pp. 173-178.

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Abstract

Background: MicroRNAs are involved in key cellular processes regulating, and their misregulation is linked to cancer. The miR-302-367 cluster is exclusively expressed in embryonic stem and carcinoma cells. This cluster also promotes cell reprogramming and stemness process. In contrast, miR-145 is mostly regarded as a tumor suppressor, where it regulates cellular functions such as cell division, differentiation, and apoptosis. By suppressing the main pluripotency factors (OCT4, SOX2, MYC and KLF4), miR-145 silences the self-renewal program in ESCs. Therefore, the main aim of this study is to find a potential link between the expression level of hsa-miR-302b and hsa-miR-145 with tumor vs. non-tumor as well as-high-grade vs. low-grade states of the esophageal tissue samples. Methods: A total number of 40 formalin-fixed, paraffin-embedded (FFPE) samples of esophageal squamous-cell carcinoma (ESCC) were obtained, and the tumor and marginal non-tumor areas delineated and punched off by an expert pathologist. Total RNA-was extracted with Trizol, and cDNA synthesized using the miRCURY LNA (TM) Universal RT microRNA PCR Kit. Real-time reverse transcription polymerase chain reaction (RT-PCR) assays were performed using specific LNA-primers and SYBR Green master mix. Results: The expression level of miR-302b failed to show any significant difference, neither between tumor and their non-tumor counterparts, nor among tumors with different grades of Malignancies (p > 0.05). In contrast, miR-145 was significantly down regulated in all grades of tumor samples (p < 0.001). However, its expression level could not discriminate between different grades of malignancy (p > 0.05). Conclusion: Our data revealed a significant down-regulation of miR-145 in ESCC tissue samples. Based on our ROC curve analysis data (AUC = 0.74, p < 0.001) miR-145 could be regarded as a potential tumor marker for diagnosis of esophageal cancer.

Item Type: Article
Keywords: Esophageal cancer; FFPE; hsa-miR-302b; hsa-miR-145; molecular marker
Page Range: pp. 173-178
Journal or Publication Title: ARCHIVES OF IRANIAN MEDICINE
Abstract and Indexing: ISI, Pubmed, Scopus
Quartile : Q3
Volume: 18
Number: 3
Publisher: web of science,scopus,pubmed
Depositing User: خانم مریم زرقانی
URI: http://repository.zums.ac.ir/id/eprint/2893

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