Repository of Research and Investigative Information

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دانشگاه علوم پزشکی و خدمات بهداشتی درمانی زنجان

BDNF modifies hippocampal KCC2 and NKCC1 expression in a temporal lobe epilepsy model

(2014) BDNF modifies hippocampal KCC2 and NKCC1 expression in a temporal lobe epilepsy model. ACTA NEUROBIOLOGIAE EXPERIMENTALIS. pp. 276-287.

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Official URL: http://eprints2.zums.ac.ir/2891/

Abstract

Excitatory GABA actions, induced by altered expression of chloride transporters (KCC2/NKCC1), can contribute to seizure generation in temporal lobe epilepsy. In the present study, we evaluated whether BDNF administration can affect KCC2/NKCC1 expression, ictogenesis and behavioral alterations in this paradigm. Status epilepticus was induced in male rats with pilocarpine, followed by a treatment of either a single high dose or multiple injections of BDNF during the latent phase of temporal lobe epilepsy. Chloride transporters expression, spontaneous recurrent seizures, and hyperexcitability post-seizural behaviors were evaluated after treatment. NKCC1 protein expression was markedly upregulated, whereas that of KCC2 was significantly downregulated in epileptic hippocampi compared to intact controls. Application of BDNF (both single high dose and multiple injections) increased KCC2 expression in epileptic hippocampi, while NKCC1 expression was downregulated exclusively by the single high dose injection of BDNF. Development of spontaneous recurrent seizures was delayed but not prevented by the treatment, and hyperexcitability behaviors were ameliorated for a short period of time. To prevent GABA-A mediated depolarization and design appropriate treatment strategies for temporal lobe epilepsy, chloride transporters can be considered as a target. Future studies are warranted to investigate any possible therapeutic effects of BDNF via altering chloride transporters expression.

Item Type: Article
Keywords: temporal lobe epilepsy; BDNF; GABA; KCC2; NKCC1
Page Range: pp. 276-287
Journal or Publication Title: ACTA NEUROBIOLOGIAE EXPERIMENTALIS
Abstract and Indexing: ISI
Quartile : Q4
Volume: 74
Number: 3
Publisher: web of science
Depositing User: خانم مریم زرقانی
URI: http://repository.zums.ac.ir/id/eprint/1993

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