Repository of Research and Investigative Information

Repository of Research and Investigative Information

دانشگاه علوم پزشکی و خدمات بهداشتی درمانی زنجان

Molecular target therapy of AKT and NF-kB signaling pathways and multidrug resistance by specific cell penetrating inhibitor peptides in HL-60 cells

(2014) Molecular target therapy of AKT and NF-kB signaling pathways and multidrug resistance by specific cell penetrating inhibitor peptides in HL-60 cells. Asian Pacific Journal of Cancer Prevention. pp. 4353-4358.

Full text not available from this repository. (Request a copy)

Official URL: http://apps.webofknowledge.com/full_record.do?prod...

Abstract

Background: PI3/AKT and NF-kB signaling pathways are constitutively active in acute myeloid leukemia and cross-talk between the two has been shown in various cancers. However, their role in acute myeloid leukemia has not been completely explored. We therefore used cell penetrating inhibitor peptides to define the contributions of AKT and NF-kB to survival and multi drug resistance (MDR) in HL-60 cells. Materials and Methods: Inhibition of AKT and NF-kB activity by AKT inhibitor peptide and NBD inhibitor peptide, respectively, resulted in decreased expression of mRNA for the MDR1 gene as assessed by real time PCR. In addition, treatment of HL-60 cells with AKT and NBD inhibitor peptides led to inhibition of cell viability and induction of apoptosis in a dose dependent manner as detected by flow cytometer. Results: Finally, co-treatment of HL-60 cells with sub-optimal doses of AKT and NBD inhibitor peptides led to synergistic apoptotic responses in AML cells. Conclusions: These data support a strong biological link between NF-kB and PI3-kinase/AKT pathways in the modulation of antiapoptotic and multi drug resistant effects in AML cells. Synergistic targeting of these pathways using NF-kB and PI3-kinase/AK inhibitor peptides may have a therapeutic potential for AML and possibly other malignancies with constitutive activation of these pathways.

Item Type: Article
Keywords: Author Keywords:AKT/PKB; NF-kB; cell penetrating peptides; AML; MDR; therapy KeyWords Plus:ACUTE MYELOID-LEUKEMIA; KAPPA-B; MAGNETIC NANOPARTICLES; DRUG-RESISTANCE; GENE-EXPRESSION; IN-VITRO; CURCUMIN; PROLIFERATION; TRANSCRIPTION; MTOR
Page Range: pp. 4353-4358
Journal or Publication Title: Asian Pacific Journal of Cancer Prevention
Abstract and Indexing: ISI, Pubmed, Scopus
Quartile : Q3
Volume: 15
Number: 10
Publisher: scopus
Depositing User: خانم مریم زرقانی
URI: http://repository.zums.ac.ir/id/eprint/1991

Actions (login required)

View Item View Item