Repository of Research and Investigative Information

Repository of Research and Investigative Information

دانشگاه علوم پزشکی و خدمات بهداشتی درمانی زنجان

The effect of silymarin (Silybum marianum) on human skin fibroblasts in an in vitro wound healing model

(2013) The effect of silymarin (Silybum marianum) on human skin fibroblasts in an in vitro wound healing model. PHARMACEUTICAL BIOLOGY. pp. 298-303.

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Abstract

Context: Silymarin, a flavonolignan from Silybum marianum (L.) Gaertn. (Asteraceae), has been reported to have antioxidant and anti-inflammatory properties. Therefore, it may be worthwhile to study the effect of silymarin on wound healing. Objective: To evaluate the effect of silymarin on human fibroblast cells in an in vitro model of wound healing. Materials and methods: Human fibroblast cells were treated with different concentrations (4.5, 9, 18, 36 mu g/mL) of silymarin. The effects of silymarin on cell viability, proliferation, collagen synthesis, and expression of cyclooxygenase-2 (COX-2) and inducible nitric oxide synthetase (iNOS) were assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, 5-bromo-2'-deoxy-uridine, hydroxyproline analysis and real-time PCR, respectively. The effect of silymarin on cellular antioxidant status was determined by protection against hydrogen peroxide (H2O2)-induced cell injury and free radical scavenging activity (ABTS assay) of the cells. Results: Results of the present study indicate that pretreatment of fibroblast cells with silymarin significantly protected cells against H2O2-induced injury (p < 0.05). After an 18 h treatment of cells with 36 mu g/mL silymarin, total antioxidant capacity of cells significantly increased (p < 0.05). Furthermore, pretreatment of human fibroblast cells with silymarin significantly inhibited lipopolysaccharide (LPS)-induced COX-2 mRNA expression (p < 0.001). There was no significant difference in fibroblast proliferation and collagen synthesis between treatment and control groups (p > 0.05). Discussion and conclusion: Silymarin may be useful as a therapeutic agent for the treatment of cutaneous wounds through its antioxidation and anti-inflammation effects.

Item Type: Article
Keywords: Author Keywords:Antioxidant capacity; collagen synthesis; cyclooxygenase-2 KeyWords Plus:FLAVONOID ANTIOXIDANT SILYMARIN; NITRIC-OXIDE-SYNTHASE; SENCAR MOUSE SKIN; OXIDATIVE STRESS; TUMOR PROMOTION; CYCLOOXYGENASE-2 EXPRESSION; HUMAN KERATINOCYTES; MICE; INHIBITION; INJURY
Page Range: pp. 298-303
Journal or Publication Title: PHARMACEUTICAL BIOLOGY
Abstract and Indexing: ISI, Scopus
Quartile : Q2
Volume: 51
Number: 3
Publisher: web of science
Depositing User: خانم مریم زرقانی
URI: http://repository.zums.ac.ir/id/eprint/1926

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