Repository of Research and Investigative Information

Repository of Research and Investigative Information

دانشگاه علوم پزشکی و خدمات بهداشتی درمانی زنجان

Toxicity of cigarette smoke on isolated lung, heart, and brain mitochondria: induction of oxidative stress and cytochrome c release

(2013) Toxicity of cigarette smoke on isolated lung, heart, and brain mitochondria: induction of oxidative stress and cytochrome c release. Toxicological and Environmental Chemistry. pp. 1624-1637.

Full text not available from this repository. (Request a copy)

Official URL: http://apps.webofknowledge.com/full_record.do?prod...

Abstract

Cigarette smoking is one of the main risk factors for premature human death which is associated with a variety of respiratory and vascular diseases, and cancer due to exposure to hundreds of toxicants. Rat mitochondria were obtained by differential ultracentrifugation and incubated with different concentrations (1%, 10%, or 100%) of standardized cigarette smoke extract (CSE). Our results showed that CSE induced a rise in mitochondrial reactive oxygen species (ROS) formation, lipid peroxidation, and mitochondrial membrane potential (MMP) collapse before mitochondrial swelling ensued in isolated pulmonary mitochondria. Disturbance in oxidative phosphorylation was also confirmed by decrease in ATP concentration in the CSE-treated mitochondria. In addition, collapse of MMP and mitochondrial swelling produced release of cytochrome c via outer membrane rupture or mitochondrial permeability transition (MPT) pore opening. Our results suggested that CSE-induced toxicity in lung tissue is the result of disruptive effect on mitochondrial respiratory chain that leads to ROS formation, lipid peroxidation, MMP decline, and cytochrome c expulsion which results in apoptosis signaling and cell loss.

Item Type: Article
Keywords: Author Keywords:cytochrome c release; isolated mitochondria; cigarette smoking extracts (CSE); mitochondrial dysfunction KeyWords Plus:RAT-LIVER MITOCHONDRIA; IN-VITRO; TAR EXTRACTS; GENERATION; INJURY; PERMEABILITY; INFLAMMATION; EXPRESSION; EXPOSURE; VANADIUM
Page Range: pp. 1624-1637
Journal or Publication Title: Toxicological and Environmental Chemistry
Abstract and Indexing: ISI, Scopus
Quartile : Q4
Volume: 95
Number: 9
Publisher: scopus
Depositing User: خانم مریم زرقانی
URI: http://repository.zums.ac.ir/id/eprint/1832

Actions (login required)

View Item View Item