Repository of Research and Investigative Information

Repository of Research and Investigative Information

دانشگاه علوم پزشکی و خدمات بهداشتی درمانی زنجان

Preparation and characterization of gelatin nanoparticles containing pDNA encoding IL-12 and their expression in CT-26 carcinoma cells

(2013) Preparation and characterization of gelatin nanoparticles containing pDNA encoding IL-12 and their expression in CT-26 carcinoma cells. FUTURE ONCOLOGY. pp. 1195-1206.

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Abstract

Aim: Gelatin as a biodegradable, nontoxic and biocompatible natural protein is a good candidate for gene delivery. In this study, pDNA-loaded gelatin nanoparticles were prepared and characterized for the expression of the cytokine IL-12 and anti-tumor effects. Materials & methods: Gelatin-pUMVC3-hIL-12 nanoparticles were prepared by the ethanol precipitation technique and evaluated for physicochemical characteristics, cytotoxiciy and transfection efficiency. Results: The prepared particles were spherical in shape with sizes varying from 344.27 to 826.23 nm, zeta-potentials between -944 and -165 mV, and greater than 97% encapsulation efficiency. The particles were nontoxic to CT-26 carcinoma cells. The nanoparticles prepared using 0.5% gelatin solution (G14) with a mean particle size of 816.87 nm (polydispersity index = 0.56 +/- 0.01) demonstrated maximum transfection efficiency with 2.5-times higher expression compared with the naked plasmid. Conclusion: Gelatin-DNA nanoparticles using 0.5% gelatin solution had minimal cytotoxicity and can be used as a suitable candidate for further gene delivery studies and applications.

Item Type: Article
Keywords: Author Keywords:gelatin; gene delivery; IL-12; nanoparticle; plasmid DNA KeyWords Plus:CANCER GENE-THERAPY; IN-VITRO; IMMUNOGENE THERAPY; DELIVERY-SYSTEMS; CROSS-LINKING; INTERLEUKIN-12; DNA; VIVO; NANOSPHERES; IMMUNOTHERAPY
Page Range: pp. 1195-1206
Journal or Publication Title: FUTURE ONCOLOGY
Abstract and Indexing: ISI, Pubmed, Scopus
Quartile : Q3
Volume: 9
Number: 8
Publisher: web of science
Depositing User: خانم مریم زرقانی
URI: http://repository.zums.ac.ir/id/eprint/1821

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