Repository of Research and Investigative Information

Repository of Research and Investigative Information

دانشگاه علوم پزشکی و خدمات بهداشتی درمانی زنجان

Novel biotinylated chitosan-graft-polyethyleneimine copolymer as a targeted non-viral vector for anti-EGF receptor siRNA delivery in cancer cells

(2013) Novel biotinylated chitosan-graft-polyethyleneimine copolymer as a targeted non-viral vector for anti-EGF receptor siRNA delivery in cancer cells. INTERNATIONAL JOURNAL OF PHARMACEUTICS. pp. 408-416.

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Abstract

The major impediments to develop an efficient non-viral siRNA-mediated gene silencing method, as a therapeutic approach, are the low cellular uptake and intracellular delivery and release of non-viral vectors. To overcome these problems, designing a proper vector with high transfection efficiency is obviously under scrutiny of various studies. The present study, evaluate a novel biotinylated chitosan-graft-polyethyleneimine (Bio-Chi-g-PEI) copolymer as an appropriate non-viral vector for targeted delivery of siRNA to cancer cells. The composition of the synthesized Bio-Chi-g-PEI copolymer was thoroughly characterized using H-1 NMR and FTIR spectroscopy, besides the hydroxyazobenzene-2-carboxylic acid (HABA) assay. In vitro cytotoxicity assay of the Bio-Chi-g-PEI copolymers was performed by MTT assay. Cytotoxicity evaluations indicated that the new copolymer was markedly less toxic than PEI 25KD. Physicochemical properties of the Bio-Chi-g-PEI/siRNA complexes such as complex stability, size, zeta potential, and their morphology at various weight ratios, investigated by appropriate methods, revealed the suitability of the complexes for the transfection. The efficient cellular internalization of the complexes for HeLa and OVCAR-3 cells in culture media was confirmed by intracellular tracking of the prepared complexes using confocal laser scanning microscopy and Cy3-labeled anti-epidermal growth factor receptor siRNA. Finally, evaluation of the transfection efficiency and gene silencing by flow cytometry and real-time polymerase chain reaction highlighted the significantly higher efficiency of transfection and silencing for biotinylated copolymer compared with the PEI 25KD and non-biotinylated copolymer. (C) 2013 Elsevier B.V. All rights reserved.

Item Type: Article
Keywords: Author Keywords:Gene silencing; siRNA delivery; Biotinylation; Chitosan-graft-polyethyleneimine; Tumor-targeting delivery; Epidermal growth factor receptor KeyWords Plus:LOW-MOLECULAR-WEIGHT; GENE DELIVERY; TRANSFECTION EFFICIENCY; IN-VITRO; DRUG-DELIVERY; DNA DELIVERY; PHYSICOCHEMICAL PROPERTIES; RNA INTERFERENCE; COMPLEXES; NANOPARTICLES
Page Range: pp. 408-416
Journal or Publication Title: INTERNATIONAL JOURNAL OF PHARMACEUTICS
Abstract and Indexing: ISI, Pubmed, Scopus
Quartile : Q1
Volume: 456
Number: 2
Publisher: web of science
Depositing User: خانم مریم زرقانی
URI: http://repository.zums.ac.ir/id/eprint/1768

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