Repository of Research and Investigative Information

Repository of Research and Investigative Information

دانشگاه علوم پزشکی و خدمات بهداشتی درمانی زنجان

Isolation, characterization, and transplantation of bone marrow-derived cell components with hematopoietic stem cell niche properties

(2013) Isolation, characterization, and transplantation of bone marrow-derived cell components with hematopoietic stem cell niche properties. Stem Cells and Development. pp. 3052-3061.

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Abstract

Although the unique role of hematopoietic stem cell (HSC) niche in hematopoiesis has long been recognized, unsuccessful isolation of intact niche units limited their in vitro study, manipulation, and therapeutic application. Here, we isolated cell complexes based on size fractionation from mouse bone marrow (BM), characterized the derived cells, and transplanted them to irradiated mice. These cell complexes were the origin of both BM mesenchymal stem cells and various hematopoietic lineages when kept in appropriate culture conditions. They also had the potential of recruiting circulating HSC. Intraperitoneal transplantation of these structures into irradiated mice not only showed long-lasting hematopoietic multilineage reconstitution, but also could recover the stromal cells of BM. In conclusion, this study for the first time provides evidences on the feasibility and efficacy of transplantation of HSC in association with their native specialized microenvironment. As the molecular cross-talk between HSC and niche is crucial for their proper function, the proposed method could be considered as a novel hematopoietic transplantation strategy.

Item Type: Article
Keywords: KeyWords Plus:STEM/PROGENITOR CELLS; PROGENITOR CELLS; STROMAL CELLS; RICH SOURCE; COTRANSPLANTATION; IDENTIFICATION; HEMATON; CULTURE; BLOOD; MICE
Page Range: pp. 3052-3061
Journal or Publication Title: Stem Cells and Development
Abstract and Indexing: ISI, Scopus
Quartile : Q3
Volume: 22
Number: 23
Publisher: scopus
Depositing User: خانم مریم زرقانی
URI: http://repository.zums.ac.ir/id/eprint/1729

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