Repository of Research and Investigative Information

Repository of Research and Investigative Information

دانشگاه علوم پزشکی و خدمات بهداشتی درمانی زنجان

Follicular Dynamic and Immunoreactions of the Vitrified Ovarian Graft After Host Treatment with Variable Regimens of Melatonin

(2012) Follicular Dynamic and Immunoreactions of the Vitrified Ovarian Graft After Host Treatment with Variable Regimens of Melatonin. American Journal of Reproductive Immunology. pp. 401-412.

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Abstract

Problem This study investigates dose-dependent effects of melatonin on ovarian graft. Method of Study Vitrified-thawed whole ovaries of newborn mice were grafted into ovariectomized mature ones. Melatonin (20, 50, 100, and 200 mg/kg /day) was administrated to separate groups of host mice for 32 days. IgM and IgG antibodies, Th1 and Th2 cytokines, and melatonin in recipient's blood were measured. Subsequent survival of the grafted ovaries was scored. An assessment of follicular morphology was performed using TUNEL assay and hematoxylin-eosin staining. Results The administration of melatonin did not disturb the circadian rhythm of melatonin concentration. The ovarian graft lifespan was prolonged at 200 mg/kg /day melatonin (P < 0.001). However, in doses of higher than 20 mg/kg /day melatonin, the proportion of healthy follicles and ovary size decreased. Th1 cytokines levels were reduced dose dependently. However, the effect of melatonin on Th2 cytokines was not pronounced. IgM and IgG2a decreased in recipients receiving 200 mg/kg /day melatonin in comparison with non-treated group (P < 0.001), while this variables were significantly increased at the dose of 50 mg/kg /day (P < 0.001). Conclusion Melatonin at 200 mg/kg /day has an immunosuppresent effect and produce prolongation of graft survival. However, the associated reduction in healthy follicles suggests that melatonin in doses of higher than 20 mg/kg /day has no preventative ischemic action.

Item Type: Article
Keywords: Author Keywords:Antioxidant; cryopreservation; endocrinology; immunology; melatonin; ovarian transplant KeyWords Plus:RECEPTOR MESSENGER-RNA; ANTIBODY-RESPONSE; OOCYTE MATURATION; OXIDATIVE STRESS; IMMUNE-SYSTEM; PINEAL-GLAND; MOUSE OVARY; IN-VITRO; CYCLOSPORINE; SURVIVAL
Page Range: pp. 401-412
Journal or Publication Title: American Journal of Reproductive Immunology
Abstract and Indexing: ISI, Pubmed, Scopus
Quartile : Q3
Volume: 67
Number: 5
Publisher: scopus
Depositing User: خانم مریم زرقانی
URI: http://repository.zums.ac.ir/id/eprint/1614

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