Repository of Research and Investigative Information

Repository of Research and Investigative Information

دانشگاه علوم پزشکی و خدمات بهداشتی درمانی زنجان

Preparation of wormlike polymeric nanoparticles coated with silica for delivery of methotrexate and evaluation of anticancer activity against MCF7 cells

(2017) Preparation of wormlike polymeric nanoparticles coated with silica for delivery of methotrexate and evaluation of anticancer activity against MCF7 cells. Journal of Biomaterials Applications. pp. 1305-1316. ISSN 0885-3282

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Abstract

Methotrexate is one of the most effective drugs that is commonly used in the treatment of cancer. However, its application is limited due to low solubility, high toxicity and rapid metabolism. Therefore, in the present study, worm-like polymeric nanoparticles as carrier of methotrexate were prepared using biodegradable copolymers (mPEG-PCL). The impact of nanoparticles' geometry on the loading, delivery and drug's anti-cancer activity was investigated. The di-block copolymer mPEG-PCL was being synthesized by a ring opening polymerization of -caprolactone in the presence of mPEG as the initiator and Sn(oct)(2) as the catalyst. It was used for the preparation of worm-like micelles and coated with silica, so that their structures are stable after drying. The synthesized copolymers and nanoparticles were characterized by FTIR, HNMR, GPC, XRD, TGA, DLS, and FE-SEM analyses. The efficiencies of drug loading and release of nanoparticles as invitro, was studied by high performance liquid chromatography. The MTT method was used to estimate the toxicity on MCF-7 cell category. The obtained results showed that the nanoparticles were worm-like particles with less than 150nm diameter and about 1 mu m length. The loading and encapsulation efficiencies of drug by the worm-like nanoparticles were 3.5 +/- 0.14 and 65.6 +/- 0.12, respectively, while they were obtained as 2.1 +/- 0.08 and 26 +/- 0.10, respectively, for spherical nanoparticles. The methotrexate diffusional behavior of worm-like nanoparticles was compared with that of the spherical ones. On the other hand, the anti-cancer activity of MTX-loaded nanoparticles was more than the free drug. The results of the MTT assay showed strong and dose-dependent inhibition of cell (MCF-7 category) growth by the nanoparticles compared with MTX. The inhibitory concentrations (IC50 i.e. reduction viability of cell to 50) obtained for worm-like, spherical nanoparticles and free drug (incubation times 72h) were 8.25 +/- 0.20, 9.15 +/- 0.17, 12.28 +/- 0.15 mu g/mL, respectively. It can be concluded that application of non-spherical nanoparticles is a better and more effective strategy for controlled and slow release of methotrexate in the treatment of cancer.

Item Type: Article
Keywords: Worm-like nanoparticles sustained release methotrexate drug delivery IN-VIVO DELIVERY DRUG-DELIVERY COPOLYMER MICELLES BLOCK-COPOLYMER ADRIAMYCIN ARTHRITIS RELEASE SYSTEMS TUMOR SHAPE Engineering, Biomedical Materials Science, Biomaterials
Page Range: pp. 1305-1316
Journal or Publication Title: Journal of Biomaterials Applications
Abstract and Indexing: ISI, Pubmed, Scopus
Quartile : Q2
Volume: 31
Number: 9
Identification Number: https://doi.org/10.1177/0885328217698063 <Go to ISI>://WOS:000400523600006
ISSN: 0885-3282
Depositing User: خانم مریم زرقانی
URI: http://repository.zums.ac.ir/id/eprint/161

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