Repository of Research and Investigative Information

Repository of Research and Investigative Information

دانشگاه علوم پزشکی و خدمات بهداشتی درمانی زنجان

Recombinant outer membrane secretin PilQ 406-770 as a vaccine candidate for serogroup B Neisseria meningitidis

(2012) Recombinant outer membrane secretin PilQ 406-770 as a vaccine candidate for serogroup B Neisseria meningitidis. Vaccine. pp. 1710-1714.

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Abstract

Secretin PilQ is an antigenically conserved outer membrane protein which is present on most meningococci. This protein naturally expressed at high levels and is essential for meningococcal pilus expression at the cell surface. A 1095 bp fragment of C-terminal of secretin pilQ from serogroup B Neisseria meningitidis was cloned into prokaryotic expression vector pET-28a. Recombinant protein was overexpressed with IPTG and affinity-purified by Ni-NTA agarose. BALB/c mice were immunized subcutaneously with purified rPilQ(406-770) mixed with Freund's adjuvant. Serum antibody responses to serogroups A and B N. meningitidis whole cells or purified rPilQ(406-770) and functional activity of antibodies were determined by ELISA and SBA, respectively. The output of rPilQ(406-770) was approximately 50% of the total bacterial proteins. Serum IgG responses were significantly increased in immunized group with PilQ(406-770) mixed with Freund's adjuvant in comparison with control groups. Antisera produced against rPilQ(406-770) demonstrated strong surface reactivity to serogroups A and B N. meningitidis tested by whole-cell ELISA. Surface reactivity to serogroup B N. meningitidis was higher than serogroup A. The sera from PilQ(406-770) immunized animals were strongly bactericidal against serogroups A and B. These results suggest that rPilQ(406-770) is a potential vaccine candidate for serogroup B N. meningiadis. (C) 2011 Elsevier Ltd. All rights reserved.

Item Type: Article
Keywords: Author Keywords:Neisseria meningitidis; PilQ(406-770); Vaccine KeyWords Plus:SERUM-BACTERICIDAL-ANTIBODY; TRANSMISSION ELECTRON-MICROSCOPY; IV PILI; RESPONSES; PORA; STRAINS; ADULTS
Page Range: pp. 1710-1714
Journal or Publication Title: Vaccine
Abstract and Indexing: ISI, Pubmed, Scopus
Quartile : Q2
Volume: 30
Number: 9
Publisher: scopus
Depositing User: خانم مریم زرقانی
URI: http://repository.zums.ac.ir/id/eprint/1414

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