Repository of Research and Investigative Information

Repository of Research and Investigative Information

دانشگاه علوم پزشکی و خدمات بهداشتی درمانی زنجان

Direct toxicity of Rose Bengal in MCF-7 cell line: Role of apoptosis

(2009) Direct toxicity of Rose Bengal in MCF-7 cell line: Role of apoptosis. Food and Chemical Toxicology. pp. 855-859.

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Abstract

Current therapies for breast cancer are often limited by short-term efficacy due to the emergence of drug resistance. In view of this, there is much interest in the identification of new agents for the treatment of breast cancer. Rose Bengal (RB) has been used as a photosensitiser in photodynamic treatment. In the present study, we investigated the direct cytotoxic and proapoptotic effects of RB, not as a photosensitiser, in MCF-7 cells. Cell viability was quantitated by MTT assay. Apoptotic cells were determined using PI staining of DNA fragmentation by flow cytometry. Bax protein expression was studied by western blotting. ROS was measured using DCF-DA by flow cytometry analysis. The result showed RB decreased cell viability in MCF-7 cells in a concentration- and time-dependent manner. RB induced a sub-G1 peak in flow cytometry histogram of treated cells indicating apoptosis is involved in this toxicity. In Western blot analysis, Bax expression significantly increased in RB-treated cells. RB could also increase ROS production in MCF-7 cells but antioxidant GSH could not decrease the toxicity indicating this toxicity was independent of ROS production. Thus RB exerts proapoptotic effects in a MCF-7 cells and could be considered as a potential chemotherapeutic agent in breast cancer. (C) 2009 Elsevier Ltd. All rights reserved.

Item Type: Article
Keywords: Author Keywords:Rose Bengal; MCF-7; Toxicity; Apoptosis; Bax protein KeyWords Plus:ENDOPLASMIC-RETICULUM; BREAST-CANCER; THERAPY; DIFFERENTIATION; MELANOMA; TARGETS
Page Range: pp. 855-859
Journal or Publication Title: Food and Chemical Toxicology
Abstract and Indexing: ISI, Pubmed, Scopus
Quartile : Q3
Volume: 47
Number: 4
Publisher: scopus
Depositing User: خانم مریم زرقانی
URI: http://repository.zums.ac.ir/id/eprint/1076

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