Repository of Research and Investigative Information

Repository of Research and Investigative Information

دانشگاه علوم پزشکی و خدمات بهداشتی درمانی زنجان

A simple and sensitive HPLC-UV method for quantitation of lovastatin in human plasma: Application to a bioequivalence study

(2009) A simple and sensitive HPLC-UV method for quantitation of lovastatin in human plasma: Application to a bioequivalence study. Biological and Pharmaceutical Bulletin. pp. 1600-1603.

Full text not available from this repository. (Request a copy)

Official URL: http://apps.webofknowledge.com/full_record.do?prod...

Abstract

An available, simple, sensitive, and rapid method has been developed for determination of the 3-hydroxy-3-methylglutaryl (HMG)-CoA reductase inhibitor, lovastatin in human plasma. The analytical procedure involves a one-step liquid-liquid extraction method using atorvastatin as internal standard. Chromatographic separation was carried out on a reversed phase C-18 column using a mixture of 0.05 M phosphate buffer (pH 7) and acetonitrile (44.5 : 55.5, v/v) as mobile phase with UV detection set at 238 nm. The total run time of analysis was 6 min with the retention time of lovastatin being 4.3 min. A complete set of analytical method validation tests were carried out on the method. Accordingly, the method was linear in the wide range of 1-100 ng/ml. The limit of detection (LOD) and limit of quantification (LOQ) for lovastatin were 0.5 and I ng/ml, respectively. The method was shown to be precise with average within-run and between-run variations of 10.45+/-6.88 and 8.68+/-5.13%, respectively. The average within-run and between-run accuracy of the method throughout its linear range was 113.33+/-3.98 and 105.72+/-5.07%, respectively. The mean relative recovery of lovastatin from human plasma by the developed method was 88.61+/-7.00%. The applicability of the method in real pharmacokinetic situations was evaluated successfully during a bioequivalence study in 14 fasting healthy male volunteers.

Item Type: Article
Keywords: Author Keywords:lovastatin; high performance liquid chromatography; HPLC-UV; liquid-liquid extraction; bioequivalence KeyWords Plus:COA REDUCTASE INHIBITORS; MASS-SPECTROMETRY
Page Range: pp. 1600-1603
Journal or Publication Title: Biological and Pharmaceutical Bulletin
Abstract and Indexing: ISI, Scopus
Quartile : Q4
Volume: 32
Number: 9
Publisher: scopus
Depositing User: خانم مریم زرقانی
URI: http://repository.zums.ac.ir/id/eprint/1044

Actions (login required)

View Item View Item