Repository of Research and Investigative Information

Repository of Research and Investigative Information

دانشگاه علوم پزشکی و خدمات بهداشتی درمانی زنجان

Expression of recombinant alpha-1 antitrypsin in CHO and COS-7 cell lines using lentiviral vector

(2009) Expression of recombinant alpha-1 antitrypsin in CHO and COS-7 cell lines using lentiviral vector. Iranian Journal of Biotechnology. 148-156+192.

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Official URL: https://www.scopus.com/record/display.uri?eid=2-s2...

Abstract

In this study, in order to facilitate and accelerate the production of eukaryotic protein alpha 1-antitrypsin (AAT) with correct post-translational modifications, a protein production system based on the transduction of CHO and COS-7 cells using lentiviral vectors was developed. Human AAT cDNA was cloned into a replication-defective lentiviral vector. The transgene AAT-Jred chimer was transferred to CHO and COS-7 cell lines using this vector and its expressions were visualized by fluorescent microscopy. The mRNA expression levels of the AAT genes were determined using Revearse Transcriptase-Polymerase Chain Reaction (RT-PCR) and its secretion into the medium by both cell types was determined using ELISA. The results show that by employing a lentiviral vector, efficient genetic loading of CHO and COS-7 cells with the AAT gene was achieved. In conclusion, by using a Lentivirus-based gene delivery system, large amounts of recombinant human AAT protein were expressed in both CHO and COS-7 cell lines. This expression system possesses key properties that ensure its application in the delivery of therapeutic genes into mammalian cultured cells.

Item Type: Article
Keywords: alpha 1-Antitrypsin Deficiency | alpha 1-Antitrypsin | AAT deficiency
Page Range: 148-156+192
Journal or Publication Title: Iranian Journal of Biotechnology
Abstract and Indexing: Scopus
Volume: 7
Number: 3
Publisher: scopus
Depositing User: خانم مریم زرقانی
URI: http://repository.zums.ac.ir/id/eprint/1039

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